Defining the role of Drosophila lateral neurons in the control of circadian rhythms in motor activity and eclosion by targeted genetic ablation and PERIOD protein overexpression

被引:155
作者
Blanchardon, E
Grima, B
Klarsfeld, A
Chélot, E
Hardin, PE
Préat, T
Rouyer, F
机构
[1] Inst Neurobiol Alfred Fessard, CNRS, UPR 2216, NGI, F-91198 Gif Sur Yvette, France
[2] Univ Houston, Dept Biol & Biochem, Houston, TX 77204 USA
[3] DEPSN, CNRS, UPR 2197, F-91198 Gif Sur Yvette, France
关键词
circadian clock; disconnected mutant; PDF neuropeptide; tetanus toxin;
D O I
10.1046/j.0953-816x.2000.01450.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The ventral lateral neurons (LN(v)s) of the Drosophila brain that express the period (per) and pigment dispersing factor (pdf) genes play a major role in the control of circadian activity rhythms. A new P-gal4 enhancer trap line is described that is mostly expressed in the LN(v)s This P-gal4 line was used to ablate the LN(v)s by using the pro-apoptosis gene bax, to stop PER protein oscillations by overexpressing per and to block synaptic transmission with the tetanus toxin light chain (TeTxLC). Genetic ablation of these clock cells leads to the loss of robust 24-h activity rhythms and reveals a phase advance in light-dark conditions as well as a weak short-period rhythm in constant darkness. This behavioural phenotype is similar to that described for disconnected(1) (disco(1)) mutants, in which we show that the majority of the individuals have a reduced number of dorsally projecting lateral neurons which, however, fail to express PER. In both LNv-ablated and disco(1) flies, PER cycles in the so-called dorsal neurons (DNs) of the superior protocerebrum, suggesting that the weak short-period rhythm could stem from these PDF-negative cells. The overexpression of per in LNs suppresses PER protein oscillations and leads to the disruption of both activity and eclosion rhythms, indicating that PER cycling in these cells is required for both of these rhythmic behaviours. Interestingly, flies overexpressing PER in the LNs do not show any weak short-period rhythms, although PER cycles in at least a fraction of the DNs, suggesting a dominant role of the LNs on the behavioural rhythms. Expression of TeTxLC in the LN(v)s does not impair activity rhythms, which indicates that the PDF-expressing neurons do not use synaptobrevin-dependent transmission to control these rhythms.
引用
收藏
页码:871 / 888
页数:18
相关论文
共 49 条
[1]  
Boquet I, 2000, J NEUROBIOL, V42, P33
[2]   ESTABLISHMENT OF NEURONAL CONNECTIVITY DURING DEVELOPMENT OF THE DROSOPHILA LARVAL VISUAL-SYSTEM [J].
CAMPOS, AR ;
LEE, KJ ;
STELLER, H .
JOURNAL OF NEUROBIOLOGY, 1995, 28 (03) :313-329
[3]  
Cheng YZ, 1998, J NEUROSCI, V18, P741
[4]  
Deitcher DL, 1998, J NEUROSCI, V18, P2028
[5]  
DIRCKSEN H, 1987, CELL TISSUE RES, V250, P377
[6]   Molecular bases for circadian clocks [J].
Dunlap, JC .
CELL, 1999, 96 (02) :271-290
[7]   THE DISCONNECTED VISUAL-SYSTEM MUTATIONS IN DROSOPHILA-MELANOGASTER DRASTICALLY DISRUPT CIRCADIAN-RHYTHMS [J].
DUSHAY, MS ;
ROSBASH, M ;
HALL, JC .
JOURNAL OF BIOLOGICAL RHYTHMS, 1989, 4 (01) :1-27
[8]   TEMPORAL PHOSPHORYLATION OF THE DROSOPHILA PERIOD PROTEIN [J].
EDERY, I ;
ZWIEBEL, LJ ;
DEMBINSKA, ME ;
ROSBASH, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (06) :2260-2264
[9]   Rhythms of Drosophila period gene expression in culture [J].
Emery, IF ;
Noveral, JM ;
Jamison, CF ;
Siwicki, KK .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (08) :4092-4096
[10]  
EWER J, 1992, J NEUROSCI, V12, P3321