Elimination of both CD4+ and CD8+ T cells but not B cells eliminates inflammation and prolongs the survival of TGFβ1-deficient mice

Transforming growth factor beta 1 (TGF beta 1) is a potent negative immunoregulatory molecule. We have previously shown that the autoimmune-mediated weaning-age lethality of Tgfb1(-/-) mice is reversed upon genetic combination with Scid or Rag null alleles. Here, we show that elimination of T but not B cells is sufficient for the reversal, but elimination of either CD4(+) or CD8(+) cells is not. Although elimination of B cells does not rescue TGF beta 1-deficient animals from autoimmunity, B cells are hyperresponsive to LPS in the absence of TGF beta 1. TGF beta 1 deficiency leads to activation of CD8(+) T cells as suggested by down-modulation of CD8 even in the absence of CD4(+) T cells. This study provides evidence that both CD4(+) and CD8(+) T cells, but not B cells, have the ability to cause inflammation in the absence of TGF beta 1. However, though TGF beta 1-deficient B cells are hyperresponsive to stimulation, alone they are not sufficient to cause inflammation. (c) 2005 Elsevier Inc. All rights reserved.