Identification of Mendel's White Flower Character

被引:105
作者
Hellens, Roger P. [1 ]
Moreau, Carol [2 ]
Lin-Wang, Kui [1 ]
Schwinn, Kathy E. [3 ]
Thomson, Susan J. [4 ]
Fiers, Mark W. E. J. [4 ]
Frew, Tonya J. [4 ]
Murray, Sarah R. [4 ]
Hofer, Julie M. I. [2 ]
Jacobs, Jeanne M. E. [4 ]
Davies, Kevin M. [3 ]
Allan, Andrew C. [1 ]
Bendahmane, Abdelhafid [6 ]
Coyne, Clarice J. [5 ]
Timmerman-Vaughan, Gail M. [4 ]
Ellis, T. H. Noel [2 ]
机构
[1] New Zealand Inst Plant & Food Res Ltd, Auckland, New Zealand
[2] John Innes Ctr Plant Sci Res, Dept Crop Genet, Norwich NR4 7UH, Norfolk, England
[3] New Zealand Inst Plant & Food Res Ltd, Palmerston North, New Zealand
[4] New Zealand Inst Plant & Food Res Ltd, Christchurch, New Zealand
[5] Washington State Univ, USDA ARS, Western Reg Plant Intro Stn, Pullman, WA 99164 USA
[6] URGV, Evry, France
来源
PLOS ONE | 2010年 / 5卷 / 10期
关键词
SEQUENCE; PEA; ALIGNMENT; GENOME; FAMILY; GENES; EXPRESSION; EVOLUTION; GENETICS; ENCODES;
D O I
10.1371/journal.pone.0013230
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: The genetic regulation of flower color has been widely studied, notably as a character used by Mendel and his predecessors in the study of inheritance in pea. Methodology/Principal Findings: We used the genome sequence of model legumes, together with their known synteny to the pea genome to identify candidate genes for the A and A2 loci in pea. We then used a combination of genetic mapping, fast neutron mutant analysis, allelic diversity, transcript quantification and transient expression complementation studies to confirm the identity of the candidates. Conclusions/Significance: We have identified the pea genes A and A2. A is the factor determining anthocyanin pigmentation in pea that was used by Gregor Mendel 150 years ago in his study of inheritance. The A gene encodes a bHLH transcription factor. The white flowered mutant allele most likely used by Mendel is a simple G to A transition in a splice donor site that leads to a mis-spliced mRNA with a premature stop codon, and we have identified a second rare mutant allele. The A2 gene encodes a WD40 protein that is part of an evolutionarily conserved regulatory complex.
引用
收藏
页数:8
相关论文
共 36 条
[1]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[2]   A new DNA sequence assembly program [J].
Bonfield, JK ;
Smith, KF ;
Staden, R .
NUCLEIC ACIDS RESEARCH, 1995, 23 (24) :4992-4999
[3]   Legume genome evolution viewed through the Medicago truncatula and Lotus japonicus genomes [J].
Cannon, Steven B. ;
Sterck, Lieven ;
Rombauts, Stephane ;
Sato, Shusei ;
Cheung, Foo ;
Gouzy, Jerome ;
Wang, Xiaohong ;
Mudge, Joann ;
Vasdewani, Jayprakash ;
Scheix, Thomas ;
Spannagl, Manuel ;
Monaghan, Erin ;
Nicholson, Christine ;
Humphray, Sean J. ;
Schoof, Heiko ;
Mayer, Klaus F. X. ;
Rogers, Jane ;
Quetier, Francis ;
Oldroyd, Giles E. ;
Debelle, Frederic ;
Cook, Douglas R. ;
Retzel, Ernest F. ;
Roe, Bruce A. ;
Town, Christopher D. ;
Tabata, Satoshi ;
Van de Peer, Yves ;
Young, Nevin D. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (40) :14959-14964
[4]   Estimating genome conservation between crop and model legume species [J].
Choi, HK ;
Mun, JH ;
Kim, DJ ;
Zhu, HY ;
Baek, JM ;
Mudge, J ;
Roe, B ;
Ellis, N ;
Doyle, J ;
Kiss, GB ;
Young, ND ;
Cook, DR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (43) :15289-15294
[5]   Effect of 5′UTR introns on gene expression in Arabidopsis thaliana [J].
Chung, Betty Y. W. ;
Simons, Cas ;
Firth, Andrew E. ;
Brown, Chris M. ;
Hellens, Roger P. .
BMC GENOMICS, 2006, 7 (1)
[6]   Construction and characterization of two bacterial artificial chromosome libraries of pea (Pisum sativum L.) for the isolation of economically important genes [J].
Coyne, C. J. ;
McClendon, M. T. ;
Walling, J. G. ;
Timmerman-Vaughan, G. M. ;
Murray, S. ;
Meksem, K. ;
Lightfoot, D. A. ;
Shultz, J. L. ;
Keller, K. E. ;
Martin, R. R. ;
Inglis, D. A. ;
Rajesh, P. N. ;
McPhee, K. E. ;
Weeden, N. F. ;
Grusak, M. A. ;
Li, C.-M. ;
Storlie, E. W. .
GENOME, 2007, 50 (09) :871-875
[7]   Mauve: Multiple alignment of conserved genomic sequence with rearrangements [J].
Darling, ACE ;
Mau, B ;
Blattner, FR ;
Perna, NT .
GENOME RESEARCH, 2004, 14 (07) :1394-1403
[8]  
DECRESCENZI P, 1805, RURALIA COMMODA COMM, P239
[9]  
Delcher Arthur L, 2003, Curr Protoc Bioinformatics, VChapter 10, DOI 10.1002/0471250953.bi1003s00
[10]   MUSCLE: a multiple sequence alignment method with reduced time and space complexity [J].
Edgar, RC .
BMC BIOINFORMATICS, 2004, 5 (1) :1-19