5′-Deoxyribose phosphate lyase activity of human DNA polymerase ι in vitro

被引：160

作者：

Bebenek, K

Tissier, A

Frank, EG

McDonald, JP

Prasad, R

Wilson, SH

Woodgate, R

Kunkel, TA

机构：

[1] NIEHS, Genet Mol Lab, NIH, Res Triangle Pk, NC 27709 USA

[2] NIEHS, Struct Biol Lab, NIH, Res Triangle Pk, NC 27709 USA

[3] NICHHD, Sect DNA Replicat Repair & Mutagenesis, NIH, Bethesda, MD 20892 USA

来源：

SCIENCE | 2001年 / 291卷 / 5511期

关键词：

D O I：

10.1126/science.1058386

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

DNA polymerase iota (pol iota) is one of several recently discovered DNA polymerases in mammalian cells whose function is unknown. We report here that human pol iota has an intrinsic 5'-deoxyribose phosphate (dRP) Lyase activity. In reactions reconstituted with uracil-DNA glycosylase (UDC), apurinic/apyrimidinic (AP) endonuclease and DNA Ligase I, pol iota can use its dRP Lyase and polymerase activities to repair G . U and A . U pairs in DNA, These data and three distinct catalytic properties of pol iota implicate it in specialized forms of base excision repair (BER).

引用

页码：2156 / 2159

页数：4

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