Incorporation of histone H3.1 suppresses the lineage potential of skeletal muscle

被引：35

作者：

Harada, Akihito ^{[1
]}

Maehara, Kazumitsu ^{[1
]}

Sato, Yuko ^{[2
,3
]}

Konno, Daijiro ^{[4
]}

Tachibana, Taro ^{[5
]}

Kimura, Hiroshi ^{[2
,3
]}

Ohkawa, Yasuyuki ^{[1
,3
]}

机构：

[1] Kyushu Univ, Fac Med, Dept Adv Med Initiat, Fukuoka 8128582, Japan

[2] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Yokohama, Kanagawa 2268501, Japan

[3] JST, CREST, Kawaguchi, Saitama 3320021, Japan

[4] RIKEN, Ctr Dev Biol, Lab Cell Asymmetry, Kobe, Hyogo 6500047, Japan

[5] Osaka City Univ, Grad Sch Engn, Dept Bioengn, Osaka 5588585, Japan

来源：

NUCLEIC ACIDS RESEARCH | 2015年 / 43卷 / 02期

关键词：

EMBRYONIC STEM-CELLS; VARIANT H3.3; ACTIVE CHROMATIN; DEPOSITION; GENOME; GENES; STATE; DAXX; ACTIVATION; CHAPERONE;

D O I：

10.1093/nar/gku1346

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Lineage potential is triggered by lineage-specific transcription factors in association with changes in the chromatin structure. Histone H3.3 variant is thought to play an important role in the regulation of lineage-specific genes. To elucidate the function of H3.3 in myogenic differentiation, we forced the expression of GFP-H3.1 to alter the balance between H3.1 and H3.3 in mouse C2C12 cells that could be differentiated intomyotubes. GFP-H3.1 replaced H3.3 in the regulatory regions of skeletal muscle (SKM) genes and induced a decrease of H3K4 trimethylation (H3K4me3) and increase of H3K27 trimethylation (H3K27me3). Similar results were obtained by H3.3 knockdown. In contrast, MyoD-dependent H3.3 incorporation into SKM genes in fibroblasts induced an increase of H3K4me3 and H3K27me3. In mouse embryos, a bivalent modification of H3K4me3 and H3K27me3 was formed on H3.3-incorporated SKM genes before embryonic skeletal muscle differentiation. These results suggest that lineage potential is established through a selective incorporation of specific H3 variants that governs the balance of histone modifications.

引用

页码：775 / 786

页数：12

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