Developmental expression and transcriptional regulation of Ci-Pans, a novel neural marker gene of the ascidian, Ciona intestinalis

被引：8

作者：

Alfano, Christian ^{[1
]}

Russo, Monia Teresa ^{[1
]}

Spagnuolo, Antonietta ^{[1
]}

机构：

[1] Stn Zool A Dohrn, Biochem & Mol Biol Lab, I-80121 Naples, Italy

来源：

GENE | 2007年 / 406卷 / 1-2期

关键词：

Ciona; Ci-pans; nervous system; in situ hybridization; promoter; transgenesis; electroporation;

D O I：

10.1016/j.gene.2007.05.026

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A novel gene, named Ci-Pans, was isolated and characterized from the ascidian Ciona intestinalis. It is an 885-bp cDNA, is thought to encode protein with no sequence similarities to known proteins and shows a spatial and temporal specific expression pattern. In fact, besides a transient early localization in the muscle precursors, it is expressed in a dynamic fashion in the nervous system, during C. intestinalis embryogenesis, reaching very high level of expression as the development proceeds. To study Ci-Pans transcriptional control, we isolated the predicted promoter region of C. intestinalis Ci-Pans using databases for this species. Analysis of transgenic embryos, with a green fluorescence protein (GFP) reporter, showed that approximately 1 kb of the 5'-flanking sequence of the Ci-Pans gene was implicated in its specific expression in the CNS. The data on the expression pattern of Ci-Pans together with the strong activity exhibited by the 1 kb promoter region we have identified, indicate that a more deeply investigation on Ci-Pans could provide clues for exploring the complex network of nervous system-specific genes. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：36 / 41

页数：6

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