A convenient method of aligning large DNA molecules on bare mica surfaces for atomic force microscopy

被引：79

作者：

Li, JW

Bai, CL ^{[2
]}

Wang, C

Zhu, CF

Lin, Z

Li, Q

Cao, EH

机构：

[1] Chinese Acad Sci, Inst Biophys, Beijing 100171, Peoples R China

[2] Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China

来源：

NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 20期

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1093/nar/26.20.4785

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Large DNA molecules remain difficult to be imaged by atomic force microscopy (AFM) because of the tendency of aggregation. A method is described to align long DNA fibers in a single direction on unmodified mica to facilitate AFM studies. The clear background, minimal overstretching, high reproducibility and convenience of this aligning procedure make it useful for physical mapping of genome regions and the studies of DNA-protein complexes.

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收藏

页码：4785 / 4786

页数：2

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