Detection of microorganisms in vessel wall specimens of the abdominal aorta: development of a PCR assay in the absence of a gold standard

被引：23

作者：

Meijer, A

van der Vliet, JA

Schouls, LM

de Vries, A

Roholl, PJM

Ossewaarde, JM

机构：

[1] Natl Inst Publ Hlth & Environm, Res Lab Infect Dis, NL-3720 BA Bilthoven, Netherlands

[2] Natl Inst Publ Hlth & Environm, Lab Pathol & Immunobiol, NL-3720 BA Bilthoven, Netherlands

[3] Univ Nijmegen St Radboud Hosp, Dept Surg, NL-6500 HB Nijmegen, Netherlands

来源：

RESEARCH IN MICROBIOLOGY | 1998年 / 149卷 / 08期

关键词：

atherosclerosis; PCR; DNA; tissue detection; Chlamydia pneumoniae; cytomegalovirus; herpes simplex virus;

D O I：

10.1016/S0923-2508(99)80005-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A procedure in which the "Invitrogen Easy-DNA" kit was followed by a silica-based method for the isolation of DNA was developed for extraction of PCR-inhibitor-free DNA from up to 300 mg of human vessel wait tissue. Optimally designed PCR assays were developed for the detection of at least one infected cell in this amount of tissue. Details of the procedure are given for the detection of DNA of Chlamydia pneumoniae, cytomegalovirus and herpes simplex virus type 1 and type 2 in human vessel waits. The procedure can serve as a reference method or as a gold standard when a high-performance method is needed.

引用

页码：577 / 583

页数：7

共 15 条

[1] Detection of Chlamydia pneumoniae but not Helicobacter pylori in atherosclerotic plaques of aortic aneurysms [J].