Miscibility phase diagrams of giant vesicles containing sphingomyelin

被引：449

作者：

Veatch, SL

Keller, SL ^{[1
]}

机构：

[1] Univ Washington, Dept Chem, Seattle, WA 98195 USA

[2] Univ Washington, Dept Phys, Seattle, WA 98195 USA

来源：

PHYSICAL REVIEW LETTERS | 2005年 / 94卷 / 14期

关键词：

D O I：

10.1103/PhysRevLett.94.148101

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

Saturated sphingomyelin (SM) lipids are implicated in lipid rafts in cell plasma membranes. Here we use fluorescence microscopy to observe coexisting liquid domains in vesicles containing SM, an unsaturated phosphatidylcholine lipid (either DOPC or POPC), and cholesterol. We note similar phase behavior in a model membrane mixture without SM (DOPC/DPPC/Chol), but find no micron-scale liquid domains in membranes of POPC/PSM/Chol. We delineate the onset of solid phases below the miscibility transition temperature, and detail indirect evidence for a three-phase coexistence of one solid and two liquid phases.

引用

页数：4

共 31 条

[1] Cell biology - A role for lipid shells in targeting proteins to caveolae, rafts, and other lipid domains [J].