Characterization and antioxidant activity of Ginkgo biloba exocarp polysaccharides

被引：133

作者：

Chen, Jingjing ^{[1
]}

Zhang, Tao ^{[1
]}

Jiang, Bo ^{[1
]}

Mu, Wanmeng ^{[1
]}

Miao, Ming ^{[1
]}

机构：

[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2012年 / 87卷 / 01期

关键词：

Ginkgo biloba exocarp; Polysaccharide; Purification; Antioxidant activity; COMPONENTS;

D O I：

10.1016/j.carbpol.2011.06.083

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Ginkgo biloba exocarp polysaccharide (GBEP) was obtained by hot water extraction, the crude polysaccharide was deproteinized by Sevag method and fractionized by a DEAE Sepharose fast flow anion-exchange column. Five fragments were obtained, including neutral polysaccharide (GBEP-N) and four acidic polysaccharides (GBEP-A1, GBEP-A2, GBEP-A3 and GBEP-A4). GBEP-N and GBEP-A3 were further purified by Superdex 200 gel column chromatography. The resulted two fractions GBEP-NN, and GBEP-AA were characterized by FT-IR, and HPGFC (high pressure gel filtration chromatography). Monosaccharide composition was determined by RP-HPLC method of precolumn derivatization with 1-phenyl-3-5-pyrazolone. GBEP-NN was mainly composed of rhamnose, arabinose, mannose, glucose and galactose, while GBEP-AA was mainly made up of mannose, rhamnose, glucuronic acid, galacturonic acid, galactosamine, glucose, galactose, xylose, arabinose, and fucose. The crude GBEP exhibited certain antioxidant activity. At the concentration of 5 mg/mL, the hydroxyl radical scavenging effect of GBEP was 90.52%, greater than 77.37% for the positive control ascorbic acid. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：40 / 45

页数：6

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