Magnetic bead capture eliminates PCR inhibitors in samples collected from the airborne environment, permitting detection of Pneumacystis carinii DNA

被引：40

作者：

Maher, N

Dillon, HK

Vermund, SH

Unnasch, TR

机构：

[1] Univ Alabama, Div Geog Med, Birmingham, AL 35294 USA

[2] Univ Alabama, Dept Environm Hlth Sci, Birmingham, AL 35294 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2001年 / 67卷 / 01期

关键词：

D O I：

10.1128/AEM.67.1.449-452.2001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

PCR detection methods are useful in studies of organisms not amenable to culture. Inhibitors in environmental samples can interfere with such assays. We describe a magnetic bead DNA capture protocol that removes inhibitors from outdoor air samples, maintaining the sensitivity of a 16S Pneumocystis carinii mitochondrial rRNA gene-based PCR.

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收藏

页码：449 / 452

页数：4

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