Interleukin-18 up-regulates osteoprotegerin expression in stromal/osteoblastic cells

被引:62
作者
Makiishi-Shimobayashi, C
Tsujimura, T
Iwasaki, T
Yamada, N
Sugihara, A
Okamura, H
Hayashi, S
Terada, N
机构
[1] Hyogo Coll Med, Dept Pathol, Nishinomiya, Hyogo 6638501, Japan
[2] Hyogo Coll Med, Dept Otolaryngol, Nishinomiya, Hyogo 6638501, Japan
[3] Hyogo Coll Med, Inst Adv Med Sci, Host Def Lab, Nishinomiya, Hyogo 6638501, Japan
[4] Tottori Univ, Fac Med, Sch Life Sci, Dept Immunol, Yonago, Tottori 683, Japan
关键词
interleukin-18; osteoprotegerin; ST2; cell; MC3T3-E1; osteoblast; osteoclast differentiation factor; osteoclastogenesis;
D O I
10.1006/bbrc.2001.4380
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Osteoprotegerin (OPG;) and osteoclast differentiation factor (ODF) are crucial regulators of osteoclastogenesis, To determine the biological role of interleukin (IL)-18 produced by stromal/osteoblaastc cells in osteoclastogenesis, we examined the effects of IL-18 on the OPG and ODF mRNA levels in these cells. When bone marrow stromal ST2 cells, osteoblastic MC3T3-E1 cells, and mouse calvarial osteoblasts were stimulated with IL-18, the expression of OPG mRNA, but not ODF mRNA, was transiently increased, its expression reaching a maximal level at 3 h after the beginning of the culture. In accordance with this observation, all these cells expressed the mRNAs of two IL-18 receptor components and MyD88, an adapter molecule involved in IL-18 signaling. Moreover, in these cells, mitogen-activated protein kinase was phosphorylated after stimulation with IL-18. These results suggest that stromal/osteoblastic cells are IL-ls-responsive cells and that IL-18 may inhibit osteoclastogenesis by upregulating OPG expression, without stimulation of ODF production, in stromal/osteoblastic cells. (C) 2001 Academic Press.
引用
收藏
页码:361 / 366
页数:6
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