Spatial learning in male mice with different levels of aggressiveness: effects of housing conditions and nicotine administration

The main aim of the present investigation was to evaluate the possible modulation of spatial learning ability by housing conditions and level of aggressiveness in mice, also testing whether differences in locomotion and anxiety could influence this relationship. Additionally, we have examined effects of nicotine in the acquisition and retention of a spatial learning task in groups of mice differing in these variables. NMRI male mice were either group-housed or individually housed for 30 days and then classified into mice with short (SAL) and long (LAL) attack latency after a pre-screening agonistic encounter. Locomotor activity and baseline levels of anxiety of these groups were evaluated in the actimeter and elevated plus-maze. Results indicated that SAL and LAL individually housed mice displayed higher locomotion activity than LAL group-housed mice. In the plus-maze test, SAL and LAL individually housed mice showed more total and open arm entries than group-housed LAL mice, confirming the hyperactivity of individually housed mice and suggesting that isolation had no clear anxiolytic or anxiogenic actions. In the water-maze, we compared the performance of individually housed SAL, individually housed LAL mice, and group-housed LAL mice treated with nicotine (0.35 and 0.175 mg/kg) or vehicle. Nicotine did not improve acquisition in group-housed mice and even impaired it in individually housed mice. Retention of platform position was better in vehicle-treated individually housed mice in comparison with vehicle-treated group-housed mice. The present study demonstrates that housing conditions but not level of aggressiveness modify spontaneous locomotor activity and behaviors displayed on the elevated plus-maze test, and can also influence retention of a spatial learning task. (C) 2003 Elsevier Science B.V. All rights reserved.