Caveolin regulation of endothelial function

被引:235
作者
Minshall, RD
Sessa, WC
Stan, RV
Anderson, RGW
Malik, AB
机构
[1] Univ Illinois, Dept Pharmacol, Chicago Med Ctr, Chicago, IL 60612 USA
[2] Univ Illinois, Dept Anesthesiol, Chicago Med Ctr, Chicago, IL 60612 USA
[3] Yale Univ, Sch Med, Boyer Ctr Mol Med, New Haven, CT 06536 USA
[4] Univ Calif San Diego, Dept Cellular & Mol Med, La Jolla, CA 92093 USA
[5] Univ Texas, SW Med Ctr, Dept Cell Biol, Dallas, TX 75235 USA
关键词
caveolae; Src; dynamin; endothelial nitric oxide synthase; CAV1; knockout; calcium; lipid raft domains;
D O I
10.1152/ajplung.00242.2003
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Caveolae are the sites in the cell membrane responsible for concentrating an array of signaling molecules critical for cell function. Recent studies have begun to identify the functions of caveolin-1, the 22-kDa caveolar protein that oligomerizes and inserts into the cytoplasmic face of the plasma membrane. Caveolin-1 appears to regulate caveolar internalization by stabilizing caveolae at the plasma membrane rather than controlling the shape of the membrane invagination. Because caveolin-1 is a scaffolding protein, it has also been hypothesized to function as a "master regulator" of signaling molecules in caveolae. Deletion of the caveolin-1 gene in mice resulted in cardiac hypertrophy and lung fibrosis, indicating its importance in cardiac and lung development. In the endothelium, caveolin-1 regulates nitric oxide signaling by binding to and inhibiting endothelial nitric oxide synthase ( eNOS). Increased cytosolic Ca2+ or activation of the kinase Akt leads to eNOS activation and its dissociation from caveolin-1. Caveolae have also been proposed as the vesicle carriers responsible for transcellular transport ( transcytosis) in endothelial cells. Transcytosis, the primary means of albumin transport across continuous endothelia, occurs by fission of caveolae from the membrane. This event is regulated by tyrosine phosphorylation of caveolin-1 and dynamin. As Ca2+ influx channels and pumps are localized in caveolae, caveolin-1 is also an important determinant of Ca2+ signaling in endothelial cells. Many of these findings were presented in San Diego, CA, at the 2003 Experimental Biology symposium "Caveolin Regulation of Endothelial Function" and are reviewed in this summary.
引用
收藏
页码:L1179 / L1183
页数:5
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