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High-density radiation hybrid map of human chromosome 18 and contig of 18p

被引：14
作者：
Giacalone, J
Li, X
Lehrach, H
Francke, U
机构：
[1] STANFORD UNIV,HOWARD HUGHES MED INST,SCH MED,STANFORD,CA 94305
[2] STANFORD UNIV,DEPT GENET,SCH MED,STANFORD,CA 94305
[3] MAX PLANCK INST MOL GENET,D-14195 BERLIN,GERMANY
来源：
GENOMICS | 1996年 / 37卷 / 01期
关键词：
D O I：
10.1006/geno.1996.0514
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A high-resolution radiation hybrid map of human chromosome 18 has been developed by testing DNA samples of 92 radiation hybrids (RH) from a previously characterized chromosome 18-specific RH panel. Half of the 159 STS markers were tested on RH DNA amplified by primer extension preamplification. This map includes 20 genes and 95 polymorphic markers, most of which have heterozygosity frequencies greater than 65% and, therefore, allow integration with genetic maps. The framework map consists of 137 markers ordered with odds of 1000:1 or better and spaced on average 580 kb apart. A YAC contig map of 18p was generated independently by STS content mapping of YACs using the same primers as for RH mapping. The RH map and the contig map are concordant. (C) 1996 Academic Press, Inc.
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页码：9 / 18
页数：10
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