Identification of a novel vertebrate circadian clock-regulated gene encoding the protein nocturnin

被引:120
作者
Green, CB
Besharse, JC
机构
[1] Dept. of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160-7400
关键词
D O I
10.1073/pnas.93.25.14884
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Photoreceptors of the Xenopus laevis retina are the site of a circadian clock Ar part of a differential display screen for rhythmic gene products in this system, we have identified a photoreceptor-specific mRNA expressed in peak abundance at night, cDNA cloning revealed an open reading frame encoding a putative 355 amino acid protein that we have named ''nocturnin'' (for night-factor). This protein has strong sequence similarity to the C-terminal domain of the yeast transcription factor, CCR4, as well as a leucine zipper-like dimerization motif, Nocturnin mRNA levels exhibit a high amplitude circadian rhythm and nuclear run-on analysis indicates that it is controlled by the retinal circadian clock at the level of transcription, Our observations suggest that nocturnin may function through protein-protein interaction either as a component of the circadian clock or as a downstream effector of clock function.
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页码:14884 / 14888
页数:5
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