Influence of urea on thermal denaturation of lysozyme investigated by optical and dielectric spectroscopies

被引：8

作者：

Bonincontro, A

Cinelli, S

Comaschi, T

Onori, G

机构：

[1] Univ Roma La Sapienza, Dipartimento Fis, INFM, I-00185 Rome, Italy

[2] Univ Perugia, Dipartimento Fis, INFM, I-06123 Perugia, Italy

来源：

PHYSICAL CHEMISTRY CHEMICAL PHYSICS | 2004年 / 6卷 / 05期

关键词：

D O I：

10.1039/b313117c

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Different structural probes following the unfolding process of a protein may evidence the existence of stable intermediates from the differences in the unfolding curves. In this work, we analyze the thermal unfolding of chicken egg lysozyme in the presence of 8 M urea at pH 5 combining dielectric spectroscopy in the radiofrequency region, UV absorbance and circular dichroism in the far and near UV regions. The data are consistent with a two-state model of unfolding equilibrium and do not provide support for the presence of a significantly populated intermediate state.

引用

收藏

页码：1039 / 1042

页数：4

相关论文

共 18 条

[1] MEASUREMENT OF RADIO-FREQUENCY PERMITTIVITY OF BIOLOGICAL TISSUES WITH AN OPEN-ENDED COAXIAL LINE .1. [J].

ATHEY, TW ;

STUCHLY, MA ;

STUCHLY, SS .

IEEE TRANSACTIONS ON MICROWAVE THEORY AND TECHNIQUES, 1982, 30 (01) :82-86

[2] THERMODYNAMICS OF DENATURATION OF LYSOZYME BY GUANIDINE HYDROCHLORIDE .2. DEPENDENCE ON DENATURANT CONCENTRATION AT 25 DEGREES [J].

AUNE, KC ;

TANFORD, C .

BIOCHEMISTRY, 1969, 8 (11) :4586-&

[3] Influence of pH on lysozyme conformation revealed by dielectric spectroscopy [J].

Bonincontro, A ;

De Francesco, A ;

Onori, G .

COLLOIDS AND SURFACES B-BIOINTERFACES, 1998, 12 (01) :1-5

[4] Conformational changes of lysozyme in water-ethanol mixtures [J].

Bonincontro, A ;

De Francesco, A ;

Matzeu, M ;

Onori, G ;

Santucci, A .

COLLOIDS AND SURFACES B-BIOINTERFACES, 1997, 10 (02) :105-111

[5] Conformational changes of proteins in aqueous solution induced by temperature in the pre-melting region [J].

Bonincontro, A ;

Bultrini, E ;

Calandrini, V ;

Onori, G .

PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2001, 3 (17) :3811-3813

[6] Rotational and translational dynamics of lysozyme in water-glycerol solution [J].

Bonincontro, A ;

Calandrini, V ;

Onori, G .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2001, 21 (04) :311-316

[7] Electrical conductivity and dielectric dispersion of E-coli 70S ribosomes and of 30S and 50S subunits: Effects of magnesium ions [J].

Bonincontro, A ;

Briganti, G ;

Giansanti, A ;

Pedone, F ;

Risuleo, G .

COLLOIDS AND SURFACES B-BIOINTERFACES, 1996, 6 (03) :219-226

[8] Effect of trehalose on alkaline transition of cytochrome-c [J].

Bonincontro, A ;

Bultrini, E ;

Calandrini, V ;

Cinelli, S ;

Onori, G .

JOURNAL OF PHYSICAL CHEMISTRY B, 2000, 104 (29) :6889-6893

[9] A lysozyme folding intermediate revealed by solution X-ray scattering [J].

Chen, LL ;

Hodgson, KO ;

Doniach, S .

JOURNAL OF MOLECULAR BIOLOGY, 1996, 261 (05) :658-671

[10] Effect of aqueous alcohol solutions on the thermal transition of lysozyme: A calorimetric study [J].

Cinelli, S ;

Onori, G ;

Santucci, A .

JOURNAL OF PHYSICAL CHEMISTRY B, 1997, 101 (40) :8029-8034