The occurrence of geometric polyprenol isomers in the rubber-producing plant, Eucommia ulmoides Oliver

被引：28

作者：

Bamba, T

Fukusaki, E

Kajiyama, S

Ute, K

Kitayama, T

Kobayashi, A

机构：

[1] Osaka Univ, Grad Sch Engn, Dept Biotechnol, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Grad Sch Engn Sci, Dept Chem, Toyonaka, Osaka 5608531, Japan

来源：

LIPIDS | 2001年 / 36卷 / 07期

关键词：

D O I：

10.1007/s11745-001-0778-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The chain length and geometric isomerism of polyprenols from Eucommia ulmoides Oliver were analyzed using supercritical fluid chromatography. After intensive effort to establish separation conditions for geometric isomers, a phenyl-bonded silica gel-packed column was found that cleanly separated poly-trans and -cis prenols. The presence of long-chain poly-trans prenols (> 9 mers) was confirmed for the first time in plants. Trans isomers were found in the leaf, seed coat, and root, but not in the bark and seed. Poly-trans prenols in this plant may act as intermediates for trans-polyisoprene biosynthesis.

引用

页码：727 / 732

页数：6

共 14 条

[1]

AEBI PB, 1999, BIOCHIM BIOPHYS ACTA, V1426, P239

[2] High-resolution analysis of polyprenols by supercritical fluid chromatography [J].