Preprocessing and exploratory analysis of chromatographic profiles of plant extracts

被引:72
作者
Hendriks, MMWB
Cruz-Juarez, L
De Bont, D
Hall, RD
机构
[1] Univ Utrecht, Ctr Med, Lab Metabolid Dis, NL-3584 EA Utrecht, Netherlands
[2] Plant Res Int, Wageningen, Netherlands
[3] Numico Res, Wageningen, Netherlands
关键词
metabolic profiling; metabolomics; preprocessing; alignment; weighted principal component analysis; liquid chromatography; plant extracts; Willow (Salix sp.);
D O I
10.1016/j.aca.2005.04.026
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The characterization of herbal extracts to compare samples from different origin is important for robust production and quality control strategies. This characterization is now mainly performed by analysis of selected marker compounds. Metabolic fingerprinting of full metabolite profiles of plant extracts aims at a more rapid and thorough screening or classification of plant material. We will show that HPLC is an appropriate technique for metabolic fingerprinting of secondary metabolites, given that adequate preprocessing of raw profiles is performed. Additional variation, which results from sample preparation and changing measurement conditions, usually obscures the information of interest in these raw profiles. This paper illustrates the importance of preprocessing of chromatographic fingerprinting data. Different alignment methods are discussed as well as the influence of normalization. Weighted principal component analysis is introduced as a valuable alternative to autoscaling of data. LC-UV data on Willow (Salix sp.) extracts is used to evaluate these preprocessing methods and their influence on exploratory data analysis. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:53 / 64
页数:12
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