Global metabolomic profiling targeting childhood obesity in the Hispanic population

被引：182

作者：

Butte, Nancy F. ^{[1
]}

Liu, Yan ^{[1
]}

Zakeri, Issa F. ^{[2
]}

Mohney, Robert P. ^{[3
]}

Mehta, Nitesh ^{[1
]}

Voruganti, V. Saroja ^{[4
,5
]}

Goering, Harald ^{[6
]}

Cole, Shelley A. ^{[6
]}

Comuzzie, Anthony G. ^{[6
]}

机构：

[1] Baylor Coll Med, Dept Pediat, USDA, ARS,Childrens Nutr Res Ctr, Houston, TX 77030 USA

[2] Drexel Univ, Dept Epidemiol & Biostat, Philadelphia, PA 19104 USA

[3] Metabolon Inc, Durham, NC USA

[4] Univ North Carolina Chapel Hill, Dept Nutr, Kannapolis, NC USA

[5] Univ North Carolina Chapel Hill, Inst Nutr Res, Kannapolis, NC USA

[6] Texas Biomed Res Inst, Dept Genet, San Antonio, TX USA

来源：

AMERICAN JOURNAL OF CLINICAL NUTRITION | 2015年 / 102卷 / 02期

关键词：

acylcarnitines; amino acids; carbohydrates; lipids; nucleotides;

D O I：

10.3945/ajcn.115.111872

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 [营养与食品卫生学];

摘要：

Background: Metabolomics may unravel important biological pathways involved in the pathophysiology of childhood obesity. Objectives: We aimed to 1) identify metabolites that differ significantly between nonobese and obese Hispanic children; 2) collapse metabolites into principal components (PCs) associated with obesity and metabolic risk, specifically hyperinsulinemia, hypertriglyceridemia, hyperleptinemia, and hyperuricemia; and 3) identify metabolites associated with energy expenditure and fat oxidation. Design: This trial was a cross-sectional observational study of metabolomics by using gas chromatography mass spectrometry and ultrahigh-performance liquid chromatography tandem mass spectrometry analyses performed on fasting plasma samples from 353 nonobese and 450 obese Hispanic children. Results: Branched-chained amino acids (BCAAs) (Len, Ile, and Val) and their catabolites, propionylcarnitine and butyrylcarnitine, were significantly elevated in obese children. Strikingly lower lysolipids and dicarboxylated fatty acids were seen in obese children. Steroid derivatives were markedly higher in obese children as were markers of inflammation and oxidative stress. PC6 (BCAAs and aromatic AAs) and PC10 (asparagine, glycine, and serine) made the largest contributions to body mass index, and PCIO and PC12 (acylcamitines) made the largest contributions to adiposity. Metabolic risk factors and total energy expenditure were associated with PC6, PC9 (AA and tricarboxylic acid cycle metabolites), and PC10. Fat oxidation was inversely related to PC8 (lysolipids) and positively related to PC16 (acylcamitines). Conclusions: Global metabolomic profiling in nonobese and obese children replicates the increased BCAA and acylcarnitine catabolism and changes in nucleotides, lysolipids, and inflammation markers seen in obese adults; however, a strong signature of reduced fatty acid catabolism and increased steroid derivatives may be unique to obese children. Metabolic flexibility in fuel use observed in obese children may occur through the activation of alternative intermediary pathways. Insulin resistance, hyperleptinemia, hypertriglyceridemia, hyperuricemia, and oxidative stress and inflammation evident in obese children are associated with distinct metabolomic profiles.

引用

页码：256 / 267

页数：12

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