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Isolation of Pinus radiata genomic DNA suitable for RAPD analysis
被引：25
作者：
Stange, C
Prehn, D
Arce-Johnson, P
机构：
[1] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Mol Genet & Microbiol, Santiago, Chile
[2] Pontificia Univ Catolica Chile, Fac Agron & Ingn Forestal, Dept Ciencias Recursos Nat, Santiago, Chile
关键词：
CTAB;
DNA extraction;
forest tree;
Pinus radiata;
RAPD;
D O I：
10.1023/A:1007540901981
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ;
081704 ;
摘要：
A protocol is presented for Pinus radiata genomic DNA isolation based on an alkyltrimethyl-ammonium bromide (CTAB) method described for other woody species. The method involves mortar grinding of tissue, a modified CTAB extraction employing high salt concentrations and polyvinyl pyrrolidone, a RNase A treatment and successive isoamyl alco- hol-chloroform extractions. The yield was approximately 15 μg DNA per 100 mg of initial fresh plant material. The genomic DNA obtained by this method was suitable to be used in simple sequence repeat and random-amplified-polymorphic DNA reactions. This extraction method would allow the molecular analysis of shoots from different clones within P. radiata families.
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页码：366 / 366
页数：1
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