Lubricated Biodegradable Polymer Networks for Regulating Nerve Cell Behavior and Fabricating Nerve Conduits with a Compositional Gradient

被引:15
作者
Cai, Lei [1 ]
Lu, Jie [3 ]
Sheen, Volney [3 ]
Wang, Shanfeng [1 ,2 ]
机构
[1] Univ Tennessee, Dept Mat Sci & Engn, Knoxville, TN 37996 USA
[2] Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37831 USA
[3] Harvard Univ, Sch Med, Dept Neurol, Beth Israel Deaconess Med Ctr, Boston, MA 02115 USA
基金
美国国家科学基金会;
关键词
POLY(PROPYLENE FUMARATE); SURFACE; GROWTH; FRICTION; DIFFERENTIATION; BIOMATERIALS; ADHESION; BONE;
D O I
10.1021/bm201372u
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
070307 [化学生物学]; 071010 [生物化学与分子生物学];
摘要
We present a method of tuning surface chemistry and nerve cell behavior, by photo-cross-linking methoxy poly(ethylene glycol) monoacrylate (mPEGA) with hydrophobic, semicrystalline poly(epsilon-caprolactone) diacrylate (PCLDA) at various weight compositions of mPEGA (phi(m)) from 2 to 30%. Improved surface wettability is achieved with corresponding decreases in friction, water contact angle, and capability of adsorbing proteins from cell culture media because of repulsive PEG chains tethered in the network. The responses of rat Schwann cell precursor line (SpL201), rat pheochromocytoma (PC12), and E14 mouse neural progenitor cells (NPCs) to the modified surfaces are evaluated. Nonmonotonic or parabolic dependence of cell attachment, spreading, proliferation, and differentiation on phi(m) is identified for these cell types with maximal values at phi(m) of 5-7%. In addition, NPCs demonstrate enhanced neuronal differentiated lineages on the mPEGA/PCLDA network at phi(m) of 5% with intermediate wettability and surface energy. This approach lays the foundation for fabricating heterogeneous nerve conduits with a compositional gradient along the wall thickness, which are able to promote nerve cell functions within the conduit while inhibiting cell attachment on the outer wall to prevent potential fibrous tissue formation following implantation.
引用
收藏
页码:358 / 368
页数:11
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