Pyrrolizidine alkaloid isoline-induced oxidative injury in various mouse tissues

被引：22

作者：

Liu, Tian-Yu ^{[1
]}

Chen, Ying ^{[1
]}

Wang, Zai-Yong ^{[2
]}

Ji, Li-Li ^{[1
,2
]}

Wang, Zheng-Tao ^{[1
,2
]}

机构：

[1] Shanghai Univ Tradit Chinese Med, Inst Chinese Mat Med, Shanghai Key Lab Complex Prescript, Minist Educ,Key Lab Standardizat Chinese Med, Shanghai 201203, Peoples R China

[2] Shanghai R&D Ctr Standardizat Chinese Med, Shanghai 201203, Peoples R China

来源：

EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY | 2010年 / 62卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Pyrrolizidine alkaloid; Isoline; Glutathione-S-transferase; Glutathione peroxidase; Lipid peroxidation; Catalase; CONVERTING ENZYME-ACTIVITY; MONOCROTALINE PYRROLE; METABOLISM; STRESS; LIVER; TRANSFERASES; PEROXIDATION; INDUCTION; MEMBRANE; PROTEINS;

D O I：

10.1016/j.etp.2009.04.008

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Isoline is a retronecine-type pyrrolizidine alkaloid (PA) isolated from the traditional Chinese medicinal herb Ligularia duciformis. The present investigation was carried out to evaluate isoline-induced oxidative injury in various important mouse organs. Various tissue samples were collected after mice were administrated with 100 mg/kg isoline for 36 h, and then lipid peroxidation (LPO) level, total antioxidant capacity, glutathione-S-transferase (GST), glutathione peroxidase (GPx) and catalase (CAT) activities were determined to evaluate the oxidative injury. Our results showed that the total antioxidant capacity of liver, brain and lung were all decreased after given isoline, and the LPO level was increased in liver and heart of isoline-treated mice. Further antioxidant-related enzyme activity assays showed that isoline (100 mg/kg) decreased GPx activity in liver and heart, increased CAT activity in liver, brain and heart, and decreased the GST activity in lung. Taken together, our results demonstrate that isoline can induce different oxidative injury in various important mouse organs, and of which liver is the most sensitive organ. (C) 2009 Published by Elsevier GmbH.

引用

页码：251 / 257

页数：7

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