Nonfunctionalized nanocrystals can exploit a cell's active transport machinery delivering them to specific nuclear and cytoplasmic compartments

被引:183
作者
Nabiev, Igor [1 ]
Mitchell, Siobhan
Davies, Anthony
Williams, Yvonne
Kelleher, Dermot
Moore, Richard
Gun'ko, Yurii K.
Byrne, Stephen
Rakovich, Yury P.
Donegan, John F.
Sukhanova, Alyona
Conroy, Jennifer
Cottell, David
Gaponik, Nikolai
Rogach, Andrey
Volkov, Yuri
机构
[1] Univ Reims, EA No Detect & Approaches Therapeut NMBD 3798, F-51100 Reims, France
[2] Trinity Coll Dublin, CRANN Res Ctr, Dublin 2, Ireland
[3] Natl Univ Ireland Univ Coll Dublin, Dublin 4, Ireland
[4] Tech Univ Dresden, D-01069 Dresden, Germany
[5] Univ Munich, Ctr NanoSci, D-80539 Munich, Germany
关键词
D O I
10.1021/nl0719832
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We use high content cell analysis, live cell fluorescent imaging, and transmission electron microscopy approaches combined with inhibitors of cellular transport and nuclear import to conduct a systematic study of the mechanism of interaction of nonfunctionalized quantum dots (QDs) with live human blood monocyte-derived primary macrophages and cell lines of phagocytic, epithelial, and endothelial nature. Live human macrophages are shown to be able to rapidly uptake and accumulate ON in distinct cellular compartment specifically to ON size and charge. We show that the smallest QDs specifically target histories in cell nuclei and nucleoli by a multistep process involving endocytosis, active cytoplasmic transport, and entering the nucleus via nuclear pore complexes. Treatment of the cells with an anti-microtubule agent nocodazole precludes ON cytoplasmic transport whereas a nuclear import inhibitor thapsigargin blocks OD import into the nucleus. These results demonstrate that the nonfunctionalized QDs exploit the cell's active transport machineries for delivery to specific intranuclear destinations.
引用
收藏
页码:3452 / 3461
页数:10
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