T4 DNA ligase synthesizes dinucleoside polyphosphates

被引：34

作者：

Madrid, O ^{[1
]}

Martín, D ^{[1
]}

Atencia, EA ^{[1
]}

Sillero, A ^{[1
]}

Sillero, MAG ^{[1
]}

机构：

[1] Univ Autonoma Madrid, Fac Med, CSIC, Inst Invest Biomed,Dept Bioquim, Madrid 29029, Spain

来源：

FEBS LETTERS | 1998年 / 433卷 / 03期

关键词：

Ap(3)A; Ap(4)A; p(4)A; FHIT gene; T4 DNA ligase; dinucleoside polyphosphate;

D O I：

10.1016/S0014-5793(98)00932-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

T4 DNA ligase (EC 6.5,1.1), one of the most widely used enzymes in genetic engineering, transfers AMP from the AMP complex to tripolyphosphate, ADP, ATP, GTP or dATP producing p(4)A, Ap(3)A, Ap(4)A, Ap(4)G and Ap(4)dA, respectively. Nicked DNA competes very effectively with GTP for the synthesis of Ap(4)G and, conversely, tripolyphosphate (or GTP) inhibits the ligation of DNA by the ligase, As T4 DNA ligase has similar requirements for ATP as the mammalian DNA ligase(s), the latter enzyme(s) could also synthesize dinucleoside polyphosphates, The present report may be related to the recent finding that human Fhit (fragile histidine triad) protein, encoded by the FHIT putative tumor suppressor gene, is a typical dinucleoside 5',5 "-P-1,P-3-triphosphate (Ap(3)A) hydrolase (EC 3.6,1.29), (C) 1998 Federation of European Biochemical Societies.

引用

页码：283 / 286

页数：4

共 42 条

[1] PRIMARY STRUCTURE AND GENETIC ORGANIZATION OF PHAGE-T4 DNA-LIGASE [J].

ARMSTRONG, J ;

BROWN, RS ;

TSUGITA, A .

NUCLEIC ACIDS RESEARCH, 1983, 11 (20) :7145-7156

[2] Fhit, a putative tumor suppressor in humans, is a dinucleoside 5',5'''-P-1,P-3-triphosphate hydrolase [J].

Barnes, LD ;

Garrison, PN ;

Siprashvili, Z ;

Guranowski, A ;

Robinson, AK ;

Ingram, SW ;

Croce, CM ;

Ohta, M ;

Huebner, F .

BIOCHEMISTRY, 1996, 35 (36) :11529-11535

[3] DIADENOSINE POLYPHOSPHATES - THEIR BIOLOGICAL AND PHARMACOLOGICAL SIGNIFICANCE [J].

BAXI, MD ;

VISHWANATHA, JK .

JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS, 1995, 33 (03) :121-128

[4] Crystal structures of HINT demonstrate that histidine triad proteins are GalT-related nucleotide-binding proteins [J].

Brenner, C ;

Garrison, P ;

Gilmour, J ;

Peisach, D ;

Ringe, D ;

Petsko, GA ;

Lowenstein, JM .

NATURE STRUCTURAL BIOLOGY, 1997, 4 (03) :231-238

[5] The past, present and future of purine nucleotides as signalling molecules [J].

Burnstock, G .

NEUROPHARMACOLOGY, 1997, 36 (09) :1127-1139

[6] DIADENOSINE TETRAPHOSPHATE IS CO-RELEASED WITH ATP AND CATECHOLAMINES FROM BOVINE ADRENAL-MEDULLA [J].

CASTILLO, CJF ;

MORO, MA ;

DELVALLE, M ;

SILLERO, A ;

GARCIA, AG ;

SILLERO, MAG .

JOURNAL OF NEUROCHEMISTRY, 1992, 59 (02) :723-732

[7] P-1,P-4-dithio-P-2,P-3-monochloromethylene diadenosine 5',5'''-P-1,P-4-tetraphosphate: A novel antiplatelet agent [J].

Chan, SW ;

Gallo, SJ ;

Kim, BK ;

Guo, MJ ;

Blackburn, GM ;

Zamecnik, PC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (08) :4034-4039

[8] Characterization of the binding of diadenosine 5',5'''-P-1,P-4-tetraphosphate (Ap(4)A) to rat liver cell membranes [J].

Edgecombe, M ;

McLennan, AG ;

Fisher, MJ .

BIOCHEMICAL JOURNAL, 1996, 314 :687-693

[9]

FONTES R, 1998, IN PRESS J BACTERIOL

[10]

Garrison P. N., 1992, AP4A OTHER DINUCLEOS, P29

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