Agrobacterium-mediated transformation of citrange:: factors affecting transformation and regeneration

被引：129

作者：

Cervera, M ^{[1
]}

Pina, JA ^{[1
]}

Juárez, J ^{[1
]}

Navarro, L ^{[1
]}

Peña, L ^{[1
]}

机构：

[1] Inst Valenciano Invest Agr, Dpto Protecc Vegetal & biotecnol, E-46113 Moncada, Spain

来源：

PLANT CELL REPORTS | 1998年 / 18卷 / 3-4期

关键词：

citrus; citrange; genetic transformation;

D O I：

10.1007/s002990050570

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The effects of cocultivation with Agrobacterium tumefaciens, regeneration and selection conditions on the transformation efficiency of citrange (Citrus sinensis L. OsbeckxPoncirus trifoliata L. Raf.) have been investigated. Factors such as cocultivation period, preculture of explants, use of acetosyringone or feeder plates during cocultivation, cocultivation on a medium rich in auxins, post cultivation in darkness, and different kanamycin concentrations for selection were assessed. A 3-day cocultivation on a medium rich in auxins improved transformation frequencies, since it increased the number of dividing cells competent for transformation, at the cut ends of the explants. Exposure of explants to darkness for 4 weeks on selection medium resulted in further callus development and increased the regeneration frequency of transgenic shoots. Furthermore, this treatment drastically reduced the number of regenerated escape shoots. A transformation efficiency of 41.3% was achieved using the optimized transformation procedure.

引用

页码：271 / 278

页数：8

共 34 条

[1] Virulence and supervirulence of Agrobacterium tumefaciens in woody fruit plants [J].

Cervera, M ;

López, MM ;

Navarro, L ;

Peña, L .

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 1998, 52 (02) :67-78

[2]

Dellaporta S.L., 1983, Plant Molecular Biology Reporter, V1, P19, DOI DOI 10.1007/BF02712670

[3]

DONG JZ, 1991, BIO-TECHNOL, V9, P858, DOI 10.1038/nbt0991-858

[4] GROWTH OF HEALTHY AND VIROID-INFECTED TOMATO CELLS IN-VITRO [J].

DURANVILA, N ;

CARBONELL, EA ;

BOADA, SP ;

SEMANCIK, JS .

PLANT SCIENCE, 1995, 105 (01) :111-120

[5] EFFICIENT TRANSFER OF A GLYPHOSATE TOLERANCE GENE INTO TOMATO USING A BINARY AGROBACTERIUM-TUMEFACIENS VECTOR [J].

FILLATTI, JJ ;

KISER, J ;

ROSE, R ;

COMAI, L .

BIO-TECHNOLOGY, 1987, 5 (07) :726-730

[6] AGROBACTERIUM MEDIATED TRANSFORMATION AND REGENERATION OF POPULUS [J].

FILLATTI, JJ ;

SELLMER, J ;

MCCOWN, B ;

HAISSIG, B ;

COMAI, L .

MOLECULAR & GENERAL GENETICS, 1987, 206 (02) :192-199

[7] Factors affecting Agrobacterium-mediated transformation in Citrus and production of sour orange (Citrus aurantium L.) plants expressing the coat protein gene of citrus tristeza virus [J].

GutierrezE, MA ;

Luth, D ;

Moore, GA .

PLANT CELL REPORTS, 1997, 16 (11) :745-753

[8] NEW AGROBACTERIUM HELPER PLASMIDS FOR GENE-TRANSFER TO PLANTS [J].

HOOD, EE ;

GELVIN, SB ;

MELCHERS, LS ;

HOEKEMA, A .

TRANSGENIC RESEARCH, 1993, 2 (04) :208-218

[9] A SIMPLE AND GENERAL-METHOD FOR TRANSFERRING GENES INTO PLANTS [J].

HORSCH, RB ;

FRY, JE ;

HOFFMANN, NL ;

EICHHOLTZ, D ;

ROGERS, SG ;

FRALEY, RT .

SCIENCE, 1985, 227 (4691) :1229-1231

[10] ACETOSYRINGONE AND OSMOPROTECTANTS LIKE BETAINE OR PROLINE SYNERGISTICALLY ENHANCE AGROBACTERIUM-MEDIATED TRANSFORMATION OF APPLE [J].

JAMES, DJ ;

URATSU, S ;

CHENG, JS ;

NEGRI, P ;

VISS, P ;

DANDEKAR, AM .

PLANT CELL REPORTS, 1993, 12 (10) :559-563

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