The effect of platelet-rich plasma on the cellular response of rat bone marrow cells in vitro

被引：58

作者：

Ogino, Y

Ayukawa, Y

Tsukiyama, Y

Koyano, K

机构：

[1] Kyushu Univ, Fac Dent Sci, Div Oral Rehabil, Sect Romovable Prosthodont,Higashi Ku, Fukuoka 8128582, Japan

[2] Kyushu Univ, Fac Dent Sci, Div Oral Rehabil, Sect Removable Prosthodont, Fukuoka 812, Japan

来源：

ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS | 2005年 / 100卷 / 03期

基金：

日本学术振兴会;

关键词：

D O I：

10.1016/j.tripleo.2005.03.007

中图分类号：

R78 [口腔科学];

学科分类号：

1003 [口腔医学];

摘要：

The aim of this study was to investigate the effect of platelet-rich plasma (PRP) on the proliferation and the differentiation of rat bone marrow cells (RBMCs). PRP, platelet-poor plasma (PPP), and bone marrow cells were derived from the rats (hearts and tibia) and the cells were cultured with or without PRP or PPP (0 [control]), 0.2 similar to 10 mu L/mL). The proliferation of RBMCs was measured on days 2 and 4, and alkaline phosphatase (ALP) staining and activity measurement were evaluated to determine the effect of PRP on the differentiation on days 4 and 8. PRP enhanced the proliferation significantly compared to the control group (P < .05). These enhancements were greater than ones induced by the addition of PPP. ALP staining appeared to show that PRP decreased the number of ALP positive cells and ALP activity significantly (P < .05). Our results demonstrate that PRP stimulates the proliferation but suppresses the differentiation of RBMCs.

引用

页码：302 / 307

页数：6

共 26 条

[1]

Anitua E, 1999, INT J ORAL MAX IMPL, V14, P529

[2]

Influence of platelet-rich plasma (PRP) on osteogenic differentiation of rat bone marrow stromal cells.: An in vitro study [J].