A multiphoton laser scanning microscope setup for transcranial in vivo brain imaging on mice

被引：12

作者：

Nase, G

Helm, PJ

Reppen, T

Ottersen, OP

机构：

[1] Univ Oslo, Ctr Mol Biol & Neurosci, Inst Basic Med Sci, Dept Anat, N-0317 Oslo, Norway

[2] Univ Oslo, Elect Lab, Inst Basic Med Sci, N-0317 Oslo, Norway

来源：

REVIEW OF SCIENTIFIC INSTRUMENTS | 2005年 / 76卷 / 12期

关键词：

The excellent help from the staff of the Mechanical Workshop; the Electronic Workshop; the Photographic Laboratory; and the IT Center of the Institute of Basic Medical Sciences; University of Oslo; is gratefully acknowledged. The multiphoton laser scan facilities and auxiliary infrastructure were financed jointly by: the National Research Council; by the University of Oslo Advanced Research Equipment Program; Medical Faculty and Institute Group of Basic Medical Sciences; by grants from Professor Letten Fegersten Saugstad’s Fund; the Jahre Foundation; and others awarded to the Laboratory of Molecular Neuroscience; and by a grant from the National Research Council to Professor Jens-Gustav Iversen;

D O I：

10.1063/1.2140487

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 [仪器科学与技术]; 080401 [精密仪器及机械]; 081102 [检测技术与自动化装置];

摘要：

We describe a multiphoton laser scanning microscope setup for transcranial in vivo brain imaging in mice. The modular system is based on a modified industrial standard Confocal Scanning Laser Microscope (CSLM) and is assembled mainly from commercially available components. A special multifunctional stage, which is optimized for both laser scanning microscopic observation and preparative animal surgery, has been developed and built. The detection unit includes a highly efficient photomultiplier tube installed in a Peltier-cooled thermal box shielding the detector from changes in room temperature and from distortions caused by external electromagnetic fields. The images are recorded using a 12-bit analog-to-digital converter. Depending on the characteristics of the staining, individual nerve cells can be imaged down to at least 100 mu m below the intact cranium and down to at least 200 mu m below the opened cranium. (c) 2005 American Institute of Physics.

引用

页码：1 / 5

页数：5

共 9 条

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[2]

Growth arrest of individual senile plaques in a model of Alzheimer's disease observed by in vivo multiphoton microscopy [J].