ActA and human zyxin harbour Arp2/3-independent actin-polymerization activity

被引：99

作者：

Fradelizi, J

Noireaux, V

Plastino, J

Menichi, B

Louvard, D

Sykes, C

Golsteyn, RM

Friederich, E

机构：

[1] Inst Curie, Lab Morphogenese & Signalisat Cellulaires, CNRS, UMR 144, F-75248 Paris 05, France

[2] Inst Curie, Lab Physicochim Curie, CNRS, UMR 168, F-75248 Paris, France

[3] Ctr Univ, CRP Sante, CNRS, Lab Franco Luxembourgeois Rech Biomed, L-1511 Luxembourg, Luxembourg

来源：

NATURE CELL BIOLOGY | 2001年 / 3卷 / 08期

关键词：

D O I：

10.1038/35087009

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The actin cytoskeleton is a dynamic network that is composed of a variety of F-actin structures. To understand how these structures are produced, we tested the capacity of proteins to direct actin polymerization in a bead assay in vitro and in a mitochondrial-targeting assay in cells. We found that human zyxin and the related protein ActA of Listeria monocytogenes can generate new actin structures in a vasodilator-stimulated phosphoprotein-dependent (VASP) manner, but independently of the Arp2/3 complex. These results are consistent with the concept that there are multiple actin-polymerization machines in cells. With these simple tests it is possible to probe the specific function of proteins or identify novel molecules that act upon cellular actin polymerization.

引用

页码：699 / 707

页数：9

共 45 条

[1] Relationship between Arp2/3 complex and the barbed ends of actin filaments at the leading edge of carcinoma cells after epidermal growth factor stimulation [J].