An improved method for the analysis of sennosides in Cassia angustifolia by high-performance liquid chromatography

被引：18

作者：

Bala, S ^{[1
]}

Uniyal, GC ^{[1
]}

Dubey, T ^{[1
]}

Singh, SP ^{[1
]}

机构：

[1] CIMAP, Lucknow 226015, Uttar Pradesh, India

来源：

PHYTOCHEMICAL ANALYSIS | 2001年 / 12卷 / 04期

关键词：

HPLC; sennosides; Cassia angustifolia;

D O I：

10.1002/pca.586

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reversed-phase column liquid chromatographic method for the analysis of sennosides A and B present in leaf and pod extracts of Cassia angustifolia has been developed using a Symmetry Cls column and a linear binary gradient profile. The method can be utilised for the quantitative determination of other sennosides as a baseline resolution for most of the constituents was achieved, The method is economical in terms of the time taken and the amount of solvent used (25 mt) for each analysis. The validity of the method with respect to analysis was confirmed by comparing the UV spectra of each peak with those of reference compounds using a photodiode array detector. Copyright (C) 2001 John Wiley & Sons, Ltd.

引用

页码：277 / 280

页数：4

共 12 条

[1] 2-DIMENSIONAL COLUMN LIQUID-CHROMATOGRAPHIC TECHNIQUE FOR RESOLUTION OF COMPLEX-MIXTURES [J].