Highly efficient biocatalysts via covalent immobilization of Candida rugosa lipase on ethylene glycol-modified gold-silica nanocomposites

被引:36
作者
Drechsler, U [1 ]
Fischer, NO [1 ]
Frankamp, BL [1 ]
Rotello, VM [1 ]
机构
[1] Univ Massachusetts, Dept Chem, Amherst, MA 01003 USA
关键词
D O I
10.1002/adma.200306022
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The polymer-mediated self-assembly of silica and gold nanoparticles (see inset in Figure and cover) leads to extended aggregates. After calcination and subsequent surface modification, these aggregates serve as supports for the covalent immobilization of lipase (see Scheme). The obtained biocatalysts exhibit high efficiencies and long-term stability.
引用
收藏
页码:271 / +
页数:5
相关论文
共 36 条
  • [1] Alcantar NA, 2000, J BIOMED MATER RES, V51, P343, DOI 10.1002/1097-4636(20000905)51:3<343::AID-JBM7>3.0.CO
  • [2] 2-D
  • [3] P(AAm-co-EG) interpenetrating polymer networks grafted to oxide surfaces: Surface characterization, protein adsorption, and cell detachment studies
    Bearinger, JP
    Castner, DG
    Golledge, SL
    Rezania, A
    Hubchak, S
    Healy, KE
    [J]. LANGMUIR, 1997, 13 (19) : 5175 - 5183
  • [4] Novel developments in biocatalytic organic chemistry
    Bertau, M
    [J]. CURRENT ORGANIC CHEMISTRY, 2002, 6 (11) : 987 - 1014
  • [5] Boal AK, 2001, ADV FUNCT MATER, V11, P461, DOI 10.1002/1616-3028(200112)11:6<461::AID-ADFM461>3.0.CO
  • [6] 2-0
  • [7] Immobilizing enzymes: How to create more suitable biocatalysts
    Bornscheuer, UT
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (29) : 3336 - 3337
  • [8] Optimizing lipases and related enzymes for efficient application
    Bornscheuer, UT
    Bessler, C
    Srinivas, R
    Krishna, SH
    [J]. TRENDS IN BIOTECHNOLOGY, 2002, 20 (10) : 433 - 437
  • [9] Carrea G, 2000, ANGEW CHEM INT EDIT, V39, P2226
  • [10] Mediated biosensors
    Chaubey, A
    Malhotra, BD
    [J]. BIOSENSORS & BIOELECTRONICS, 2002, 17 (6-7) : 441 - 456