Apc10.1:: An Apcmin/+ intestinal cell line with retention of heterozygosity

APC10.1 is a new intestinal cell line derived from Apc(Min/+) mice that retains both the heterozygous Apc genotype and a nonactivated Wnt signaling pathway and displays an early neoplastic phenotype. Although tumorigenic both in immunodepressed and in immunocompetent syngeneic mice, it requires a high cell dose and a long latency. Its epithelial/ intestinal origin is shown, in a gene expression profile, by the expression of epithelial transcripts (such as cytokeratin and laminin isoforms) and of developmental regulatory genes (such as Tcf-4, Hnf3beta, p21, 1hh, Hes1) necessary for, or involved in, the maintenance of intestinal stem cells. The lack of activation of the Writ cascade in APC10.1 cells is shown both by the expression profile of Writ target genes and by the standard TCF reporter assay. APC10.1 cell line is a novel in vitro model that can contribute to a better understanding of the clinical evolution of familial adenomatous polyposis and to finding of new prophylactic and therapeutic approaches. Supplementary material for this article can be found on the International Joumal of Cancer website at http://www.interscience.wiley.com/ipages/0020-7136/suppmatlindex.htmi. (C) 2003 Wiley-Liss, Inc.