Monitoring the susceptibility to oseltamivir of Influenza A(H1N1) 2009 virus by nested-PCR and pyrosequencing during the pandemic and in the season 2010-2011

被引：7

作者：

Arvia, Rosaria ^{[1
]}

Corcioli, Fabiana ^{[1
]}

Simi, Lisa ^{[2
]}

Orlando, Claudio ^{[2
]}

De Santis, Riccardo ^{[1
]}

Facchini, Marzia ^{[3
]}

Donatelli, Isabella ^{[3
]}

Azzi, Alberta ^{[1
]}

机构：

[1] Univ Florence, Dept Publ Hlth, I-50134 Florence, Italy

[2] Univ Florence, Dept Clin Physiopathol, I-50134 Florence, Italy

[3] Natl Inst Hlth, Natl Influenza Ctr, Rome, Italy

来源：

JOURNAL OF VIROLOGICAL METHODS | 2012年 / 184卷 / 1-2期

关键词：

Antiviral resistance; Influenza virus; Pyrosequencing; Nested PCR; RESISTANCE;

D O I：

10.1016/j.jviromet.2012.05.018

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

For the early detection of the H275Y mutation as a marker of oseltamivir resistance in A(H1N1) pandemic strains, a sensitive and specific pyrosequencing assay was developed. This assay analyses a region 99 nts long, encompassing the H275Y site, amplified by a nested PCR. Seventy-five respiratory specimens, obtained from 62 patients during the pandemic and in the 2010-2011 influenza season, in Tuscany, were tested. Resistant strains were demonstrated in 10 patients. In three other patients, resistant and sensitive variants were found. This pyrosequencing assay may be a useful method for monitoring the spread of resistant influenza H1N1 2009 strains. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：113 / 116

页数：4

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