The chemoenzymatic synthesis of the core trisaccharide of N-linked oligosaccharides using a recombinant β-mannosyltransferase

被引：30

作者：

Watt, GM ^{[1
]}

Revers, L ^{[1
]}

Webberley, MC ^{[1
]}

Wilson, IBH ^{[1
]}

Flitsch, SL ^{[1
]}

机构：

[1] Univ Edinburgh, Dept Chem, Edinburgh Ctr Prot Technol, Edinburgh EH9 3JJ, Midlothian, Scotland

来源：

CARBOHYDRATE RESEARCH | 1997年 / 305卷 / 3-4期

基金：

英国生物技术与生命科学研究理事会;

关键词：

N-linked oligosaccharides; beta-mannosyltransferase;

D O I：

10.1016/S0008-6215(97)00261-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The chemical synthesis of the beta-mannosyl linkage of N-glycans has presented a great challenge to synthetic carbohydrate chemists. We have therefore investigated the application of beta-mannosyltransferases to the preparative synthesis N-linked core oligosaccharides. In this paper we report the chemoenzymatic synthesis of beta-D-mannopyranosyl-(1-->4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-->4)-2-acetamido-2-deoxy-alpha-D-glucopyranose on a preparative scale using a phytanyl-linked acceptor in the presence of a recombinant beta-(1-->4)-mannosyltransferase. (C) 1998 Elsevier Science Ltd.

引用

页码：533 / 541

页数：9

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