Determination of 1,4-benzodiazepines by high-performance liquid chromatography electrospray tandem mass spectrometry

被引：52

作者：

Kleinschnitz, M ^{[1
]}

Herderich, M ^{[1
]}

Schreier, P ^{[1
]}

机构：

[1] UNIV WURZBURG,LEHRSTUHL LEBENSMITTELCHEM,D-97074 WURZBURG,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1996年 / 676卷 / 01期

关键词：

1,4-benzodiazepines; diazepam; N-desmethyldiazepam; nitrazepam; flunitrazepam; medazepam;

D O I：

10.1016/0378-4347(95)00412-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive and selective method to determine diazepam, N-desmethyldiazepam, nitrazepam, flunitrazepam and medazepam in human serum and urine was established employing solid-phase extraction and high-performance liquid chromatography-electrospray tandem mass spectrometry (HPLC-ESI-MS-MS) with the selected reaction monitoring (SRM) mode. Using diazepam-d(5) and N-desmethyldiazepam-d(5) as internal standards, recoveries between 90.4 and 109.7% were determined. The routine quantification limit was set at 2 ng/ml for serum and urine at a signal-to-noise ratio of 10:1.

引用

页码：61 / 67

页数：7

共 19 条

[1] DETERMINATION OF BENZODIAZEPINES BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY [J].

BECHET, I ;

FILLET, M ;

HUBERT, P ;

CROMMEN, J .

ELECTROPHORESIS, 1994, 15 (10) :1316-1321

[2] FRAGMENTATION OF 5-PHENYL-1,4-BENZODIAZEPIN-2-ONES - MECHANISM FOR THE FORMATION OF [M-H]+ [J].

BENZ, W ;

VANE, FM ;

RAPP, U .

ORGANIC MASS SPECTROMETRY, 1979, 14 (03) :154-159

[3] URINARY SCREENING FOR BENZODIAZEPINES WITH RADIORECEPTOR ASSAY - COMPARISON WITH EMIT(R) DAU BENZODIAZEPINE ASSAY AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

BORGGAARD, B ;

JOERGENSEN, I .

JOURNAL OF ANALYTICAL TOXICOLOGY, 1994, 18 (05) :243-246

[4] DETERMINATION OF 1,4-BENZODIAZEPINES AND 1,4-BENZODIAZEPINE-2-ONES IN BLOOD BY ELECTRON-CAPTURE GAS-LIQUID-CHROMATOGRAPHY [J].

DESILVA, JAF ;

BEKERSKY, I ;

PUGLISI, CV ;

BROOKS, MA ;

WEINFELD, RE .

ANALYTICAL CHEMISTRY, 1976, 48 (01) :10-19

[5] QUANTITATION BY GAS-CHROMATOGRAPHY WITH SELECTED-ION MONITORING MASS-SPECTROMETRY OF NATURAL DIAZEPAM, N-DESMETHYLDIAZEPAM AND OXAZEPAM IN NORMAL HUMAN SERUM [J].

DUTHEL, JM ;

CONSTANT, H ;

VALLON, JJ ;

ROCHET, T ;

MIACHON, S .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1992, 579 (01) :85-91

[6]

EBEL S, 1977, DTSCH APOTH ZTG, V40, P1605

[7] DETERMINATION OF L-365,260, A NEW CHOLECYSTOKININ RECEPTOR (CCK-B) ANTAGONIST, IN PLASMA BY LIQUID-CHROMATOGRAPHY ATMOSPHERIC-PRESSURE CHEMICAL IONIZATION MASS-SPECTROMETRY [J].

GILBERT, JD ;

HAND, EL ;

YUAN, AS ;

OLAH, TV ;

COVEY, TR .

BIOLOGICAL MASS SPECTROMETRY, 1992, 21 (02) :63-68

[8] DETERMINATION OF BENZODIAZEPINE RESIDUES IN BIOLOGICAL TISSUES AND FLUIDS BY GAS-LIQUID-CHROMATOGRAPHY [J].

HOHNE, D ;

BOHN, G ;

THIER, HP .

FRESENIUS ZEITSCHRIFT FUR ANALYTISCHE CHEMIE, 1987, 328 (1-2) :99-104

[9]

JONES CE, 1989, CLIN CHEM, V35, P1394

[10] SCREENING, IDENTIFICATION, AND QUANTITATION OF BENZODIAZEPINES IN POSTMORTEM SAMPLES BY HPLC WITH PHOTODIODE-ARRAY DETECTION [J].

LAMBERT, WE ;

MEYER, E ;

XUEPING, Y ;

DELEENHEER, AP .

JOURNAL OF ANALYTICAL TOXICOLOGY, 1995, 19 (01) :35-40

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