Epidermal growth factor, but not hepatocyte growth factor, suppresses the apoptosis induced by transforming growth factor-beta in fetal hepatocytes in primary culture

被引:70
作者
Fabregat, I
Sanchez, A
Alvarez, AM
Nakamura, T
Benito, M
机构
[1] UNIV COMPLUTENSE MADRID,CTR CITOMETRIA FLUJO & MICROSCOPIA CONFOCAL,E-28040 MADRID,SPAIN
[2] OSAKA UNIV,SCH MED,BIOMED RES CTR,SUITA,OSAKA 565,JAPAN
关键词
TGF-beta; apoptosis; fetal hepatocyte; HGF; EGF;
D O I
10.1016/0014-5793(96)00266-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We studied whether the TGF-beta-induced apoptosis in fetal hepatocyte primary cultures may be modulated by the presence of mitogenic stimuli, such as EGF or HGF, EGF prevented cell death, shelving a dose dependence that was identical to that observed for its effect on DNA synthesis stimulation, HGF, in contrast, had no effect, even at high concentrations, EGF blocked apoptosis, since in the presence of this factor cells did not show DNA fragmentation, Moreover, EGF, but not HGF, blocked c-fos induction associated with the apoptotic process induced by TGF-beta in these cells.
引用
收藏
页码:14 / 18
页数:5
相关论文
共 30 条
[1]  
BENITO MD, 1992, EXP CELL RES, V202, P495
[2]   TGF-BETA GENE-TRANSCRIPTION IN NORMAL AND NEOPLASTIC LIVER GROWTH [J].
CARR, BI ;
HUANG, TH ;
ITAKURA, K ;
NOEL, M ;
MARCEAU, N .
JOURNAL OF CELLULAR BIOCHEMISTRY, 1989, 39 (04) :477-487
[3]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[4]  
COLUMBANO A, 1985, LAB INVEST, V52, P670
[5]   HEPATOCYTE GROWTH-FACTOR UP-REGULATES MET EXPRESSION IN RAT FETAL HEPATOCYTES IN PRIMARY CULTURE [J].
DEJUAN, C ;
SANCHEZ, A ;
NAKAMURA, T ;
FABREGAT, I ;
BENITO, M .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 204 (03) :1364-1370
[6]  
DRYSDALE BE, 1983, J IMMUNOL, V131, P2362
[7]   TRANSFORMING GROWTH-FACTOR BETA-MODULATES THE EXPRESSION OF COLLAGENASE AND METALLOPROTEINASE INHIBITOR [J].
EDWARDS, DR ;
MURPHY, G ;
REYNOLDS, JJ ;
WHITHAM, SE ;
DOCHERTY, AJP ;
ANGEL, P ;
HEATH, JK .
EMBO JOURNAL, 1987, 6 (07) :1899-1904
[8]   GROWTH-STIMULATION OF RAT FETAL HEPATOCYTES IN RESPONSE TO HEPATOCYTE GROWTH-FACTOR - MODULATION OF C-MYC AND C-FOS EXPRESSION [J].
FABREGAT, I ;
DEJUAN, C ;
NAKAMURA, T ;
BENITO, M .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 189 (02) :684-690
[9]   TGF-BETA IN LIVER DEVELOPMENT, REGENERATION, AND CARCINOGENESIS [J].
FAUSTO, N ;
MEAD, JE ;
GRUPPUSO, PA ;
BRAUN, L .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1990, 593 :231-242
[10]   INDUCTION OF APOPTOSIS BY TRANSFORMING GROWTH-FACTOR-BETA-1 IN THE RAT HEPATOMA-CELL LINE MCA-RH7777 - A POSSIBLE ASSOCIATION WITH TISSUE TRANSGLUTAMINASE EXPRESSION [J].
FUKUDA, K ;
KOJIRO, M ;
CHIU, JF .
HEPATOLOGY, 1993, 18 (04) :945-953