A genetic approach to in vitro regeneration of non regenerating cotton (Gossypium hirsutum L.) cultivars

被引：41

作者：

Kumar, S ^{[1
]}

Sharma, P ^{[1
]}

Pental, D ^{[1
]}

机构：

[1] Univ Delhi, Dept Genet, New Delhi 110021, India

来源：

PLANT CELL REPORTS | 1998年 / 18卷 / 1-2期

关键词：

cotton; Gossypium hirsutum; somatic embryogenesis;

D O I：

10.1007/s002990050532

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Selections were made among individual plants of Gossypium hirsutum cv 'Coker 310' for high-frequency in vitro regeneration by somatic embryogenesis. After three generations of selection, a pure line for high-frequency somatic embryogenesis was selected and named Coker 310 FR (FR, fully regenerating). Coker 310FR could be regenerated by following previously published protocols (see Materials and methods) and a modified protocol developed in this study that reduced the time necessary for in vitro regeneration. Coker 310FR was crossed with individual plants of major cotton cultivars grown in India, namely 'MCU 5', 'MCU 7', 'Khandwa 2', 'Bikaneri Nerma', 'F846' that have been shown to be recalcitrant to in vitro regeneration, to evaluate the regeneration potential of F(1)s. All the F(1)s showed regeneration by somatic embryogenesis. However, the F-1 of G. barbadense x G. hirsutum Coker 310FR did not regenerate.

引用

页码：59 / 63

页数：5

共 14 条

[1]

Basu A. K., 1988, Journal of the Indian Society for Cotton Improvement, V13, P79

[2] PLANT-REGENERATION FROM CALLUS-TISSUE OF GOSSYPIUM-HIRSUTUM-L [J].