Molecular detection and identification of agents of eumycetoma: Detailed report of two cases

被引:38
作者
Ahmed, AOA
Desplaces, N
Leonard, P
Goldstein, F
De Hoog, S
Verbrugh, H
van Belkum, A
机构
[1] Erasmus Univ, Ctr Med, Dept Med Microbiol & Infect Dis, NL-3015 GD Rotterdam, Netherlands
[2] Univ Khartoum, Inst Endem Dis, Mycetoma Res Grp, Khartoum, Sudan
[3] Cent Bur Schimmelcultures, Utrecht, Netherlands
[4] Croix St Simon Hosp, Dept Microbiol, Paris, France
[5] Croix St Simon Hosp, Dept Orthoped Surg, Paris, France
[6] St Joseph Hosp, Dept Microbiol, Paris, France
关键词
D O I
10.1128/JCM.41.12.5813-5816.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We describe two cases of eumycetoma in the legs. The infections could not be adequately diagnosed by classical mycology, but the causative agents were successfully identified as Madurella mycetomatis by species-specific PCR and DNA sequencing.
引用
收藏
页码:5813 / 5816
页数:4
相关论文
共 13 条
  • [1] Development of a species-specific PCR-restriction fragment length polymorphism analysis procedure for identification of Madurella mycetomatis
    Ahmed, AOA
    Mukhtar, MM
    Kools-Sijmons, M
    Fahal, AH
    de Hoog, S
    Van den Ende, BG
    Zijlstra, EE
    Verbrugh, H
    Abugroun, ESAM
    Elhassan, AM
    van Belkum, A
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (10) : 3175 - 3178
  • [2] Boiron P, 1998, MED MYCOL, V36, P26
  • [3] RAPID AND SIMPLE METHOD FOR PURIFICATION OF NUCLEIC-ACIDS
    BOOM, R
    SOL, CJA
    SALIMANS, MMM
    JANSEN, CL
    WERTHEIMVANDILLEN, PME
    VANDERNOORDAA, J
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (03) : 495 - 503
  • [4] BORELLI D., 1957, Bol. Lab. clin. (Caracas)., V2, P1
  • [5] BORELLI DANTE, 1962, ARCH VENEZOLANOS MED TROP PARASITOL MED, V4, P301
  • [6] de Hoog G.S., 2000, ATLAS CLIN FUNGI, VSecond
  • [7] DEHOOG GS, 1993, MYCOSES, V36, P81, DOI 10.1111/j.1439-0507.1993.tb00693.x
  • [9] Mycetomas in Mali: Causative agents and geographic distribution
    Mahe, A
    Develoux, M
    Lienhardt, C
    Keita, S
    Bobin, P
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1996, 54 (01) : 77 - 79
  • [10] Mycetoma
    McGinnis, MR
    [J]. DERMATOLOGIC CLINICS, 1996, 14 (01) : 97 - &