Duplex polymerase chain reaction (PCR) for the simultaneous detection of cryIa(b) and the maize ubiquitin promoter in the transgenic rice line KMD1

被引：7

作者：

Babekova, R. ^{[1
,2
]}

Funk, T. ^{[1
]}

Pecoraro, S. ^{[1
]}

Engel, K. -H. ^{[2
]}

Baikova, D. ^{[3
]}

Busch, U. ^{[1
]}

机构：

[1] Bavarian Hlth & Food Safety Author, Oberschleissheim, Germany

[2] Tech Univ Munich, Dept Food & Nutr, D-8050 Freising Weihenstephan, Germany

[3] Natl Ctr Publ Hlth Protect, Sofia, Bulgaria

来源：

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT | 2008年 / 22卷 / 02期

关键词：

GMOs; transgenic rice; screening; gene-specific methods; KMD1; cryIA(b); maize ubiquitin promoter; Bt11;

D O I：

10.1080/13102818.2008.10817538

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This study describes a combination of screening and gene-specific methods for the defection of GMO in food and feed Using specific primers, the maize ubiquitin promoter and the cryIA(b) gene, which encodes for a delta-endotoxin transferring insect resistance, can be detected simultaneously by a duplex PCR in samples containing the transgenic rice line Kemingdaol (KMD1). This method is also sititable for the screening for Bt11 maize.

引用

页码：705 / 708

页数：4

共 19 条

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