Nanoscale site-specific immobilization of proteins through electroactivated disulfide exchange

被引：31

作者：

Pavlovic, E

Oscarsson, S

Quist, AP

机构：

[1] Univ Uppsala, Dept Surface Biotechnol, S-75123 Uppsala, Sweden

[2] Malardalen Univ, Dept Biol & Chem Engn, S-63105 Eskilstuna, Sweden

[3] Univ Uppsala, Dept Phys, S-75121 Uppsala, Sweden

来源：

NANO LETTERS | 2003年 / 3卷 / 06期

关键词：

D O I：

10.1021/nl034191q

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new method based on the electrochemical oxidation and activation of thiols was used to generate nanoscale patterns on thiol-derivatized silicon oxide surfaces. By application of a potential difference between an AFM tip and a thiolated silicon surface, surface thiols were activated into thiolsulfinates/thiolsulfonates, which are reactive to thiol groups on biomolecules in solution. Thiol-rich protein, beta-galactosidase, was immobilized onto the patterns.

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页码：779 / 781

页数：3

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