Quantitative imaging of protein-protein interactions by multiphoton fluorescence lifetime imaging microscopy using a streak camera

被引：63

作者：

Krishnan, RV ^{[1
]}

Masuda, A ^{[1
]}

Centonze, VE ^{[1
]}

Herman, B ^{[1
]}

机构：

[1] Univ Texas, Hlth Sci Ctr, Dept Struct & Cellular Biol, San Antonio, TX 78229 USA

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2003年 / 8卷 / 03期

关键词：

fluorescence lifetime imaging microscopy;

D O I：

10.1117/1.1577574

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescence lifetime imaging microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. Recent developments in multiphoton FLIM methods are reviewed and a novel multiphoton FLIM system using a streak camera is described. An example of a typical application of the system is provided in which the fluorescence resonance energy transfer between a donor-acceptor pair of fluorescent proteins within a cellular specimen is measured. (C) 2003 Society of Photo-Optical Instrumentation Engineers.

引用

页码：362 / 367

页数：6

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