Microbial characterization of the Mars Odyssey spacecraft and its encapsulation facility

被引:136
作者
La Duc, MT
Nicholson, W
Kern, R
Venkateswaran, K
机构
[1] CALTECH, Jet Prop Lab, NASA, Biotechnol & Planetary Protect Grp, Pasadena, CA 91109 USA
[2] Univ Arizona, Dept Vet Sci & Microbiol, Tucson, AZ 85721 USA
关键词
D O I
10.1046/j.1462-2920.2003.00496.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Microbial characterization of the Mars Odyssey spacecraft and the Kennedy Space Center Spacecraft Assembly and Encapsulation Facility II (SAEF-II) was carried out by both culture-based and molecular methods. The most dominant cultivable microbes were species of Bacillus, with comamonads, microbacteria and actinomycetales also represented. Several spore-forming isolates were resistant to gamma-radiation, UV, H2O2 and desiccation, and one Acinetobacter radioresistens isolate and several Aureobasidium, isolated directly from the spacecraft, survived various conditions. Sequences arising in clone libraries were fairly consistent between the spacecraft and facility; predominant genera included Variovorax, Ralstonia and Aquaspirillum. This study improves our understanding of the microbial community structure, diversity and survival capabilities of microbes in an encapsulation facility and physically associated with colocated spacecraft.
引用
收藏
页码:977 / 985
页数:9
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