Comparative effects of cholesterol and cholesterol sulfate on hydration and ordering of dimyristoylphosphatidylcholine membranes

被引:58
作者
Faure, C
Tranchant, JF
Dufourc, EJ
机构
[1] CTR RECH PAUL PASCAL,CNRS,F-33600 PESSAC,FRANCE
[2] DIOR,CHIM PHYS LAB,F-45804 ST JEAN DE BRAYE,FRANCE
关键词
D O I
10.1016/S0006-3495(96)79696-0
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The comparative effect of cholesterol (CH) versus cholesterol sulfate (CS) on dimyristoylphosphatidylcholine (DMPC) membranes has been investigated by optical microscopy, freeze-fracture electron microscopy, x-ray diffraction, and solid state H-2 and P-31 nuclear magnetic resonance (NMR). The sulfate analogue extends the lamellar phase domain toward high water contents, and substitution of 30 mol % CH by CS in DMPC lamellae induces the trapping of 30 wt % additional water. The greater swelling of the CS-containing systems is evidenced by determination of lamellar repeat distances at maximal hydration: 147 +/- 4 Angstrom and 64 +/- 2 Angstrom in the presence of CS and CH, respectively. H-2-NMR of heavy water demonstrates that CS binds similar to 12 more water molecules at the interface than CH whereas NMR of deuterium-labeled DMPC chains reveals that 30 mol % CS orders the membrane as 15 mol % CH at high temperature and disorders much more than CH at low temperatures. The various effects of CS versus CH are discussed by taking into account attractive Van der Waals forces and repulsive steric/electrostatic interactions of the negatively charged sulfate group.
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收藏
页码:1380 / 1390
页数:11
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