Photon counting histogram for one-photon excitation

被引：33

作者：

Perroud, TD ^{[1
]}

Huang, B ^{[1
]}

Wallace, MI ^{[1
]}

Zare, RN ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

来源：

CHEMPHYSCHEM | 2003年 / 4卷 / 10期

关键词：

fluorescence; fluorescence spectroscopy; one-photon excitation; photon counting histogram; single-molecule studies;

D O I：

10.1002/cphc.200300824

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

An advance that counts! A method is presented for analyzing single-molecule behavior using one-photon excitation of fluorescence. It is based on the photon counting histogram (PCH) procedure that has enjoyed much success for two-photon excitation, but has not previously been applied to one-photon excitation. This corrected PCH model is able to resolve fluorescent species with different degrees of brightness, for example, a mixture of tetramethylrhodamine-5′-maleimide and Cy3-maleimide (see graph). Because one-photon excitation is often much easier to do than two-photon excitation, this method has the potential to be widely used and represents an important advance in the field of single-molecule spectroscopy.

引用

页码：1121 / 1123

页数：3

共 8 条

[1] The photon counting histogram in fluorescence fluctuation spectroscopy [J].