C/EBPβ Binds the P1 Promoter of the Runx2 Gene and Up-Regulates Runx2 Transcription in Osteoblastic Cells

被引：53

作者：

Henriquez, Berta ^{[1
,2
,3
,4
]}

Hepp, Matias ^{[4
]}

Merino, Paola ^{[1
,2
,3
]}

Sepulveda, Hugo ^{[1
,2
,3
,4
]}

Van Wijnen, Andre J. ^{[5
]}

Lian, Jane B. ^{[5
]}

Stein, Gary S. ^{[5
]}

Stein, Janet L. ^{[5
]}

Montecino, Martin ^{[1
,2
,3
]}

机构：

[1] Univ Andres Bello, Fac Ciencias Biol, Ctr Invest Biomed, Santiago, Chile

[2] Andres Bello Univ, Fac Biol Sci, FONDAP Ctr Genome Regulat, Santiago, Chile

[3] Andres Bello Univ, Fac Med, Ctr Biomed Res, Santiago, Chile

[4] Univ Concepcion, Fac Biol Sci, Dept Biochem & Mol Biol, Concepcion, Chile

[5] Univ Massachusetts, Sch Med, Dept Cell Biol, Worcester, MA 01655 USA

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 2011年 / 226卷 / 11期

关键词：

VITAMIN-D-RECEPTOR; PROTEIN-BETA; OSTEOCALCIN GENE; DISTAL PROMOTER; DIFFERENTIATION; EXPRESSION; ISOFORM; ELEMENT; METHYLATION; COMMITMENT;

D O I：

10.1002/jcp.22652

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

The Runx2 factor is an essential component of the regulatory mechanisms that control transcription during skeletogenesis. Runx2/p57 expression in osteoblastic cells is controlled by the P1 promoter, which is recognized by key regulators of osteoblast differentiation including homeodomain factors and Wnt- and BMP-signaling mediators. Here, we report that the transcription factor C/EBP beta up-regulates Runx2/p57 expression by directly binding to the Runx2 P1 promoter in mesenchymal, pre-osteoblastic, and osteoblastic cells. This C/EBP beta-mediated up-regulation is principally dependent on C/EBP site II that is located within the first 180 bp of the proximal P1 promoter region and is highly conserved among mouse, rat, and human Runx2 genes. Our studies reveal how the C/EBPb factor, known to have a key role during osteogenesis, contributes to regulating the expression of Runx2, the master regulator of osteoblast differentiation. J. Cell. Physiol. 226: 3043-3052, 2011. (C) 2011 Wiley-Liss, Inc.

引用

页码：3043 / 3052

页数：10

共 44 条

[1]

Reconstitution of Runx2/Cbfa1-null cells identifies a requirement for BMP2 signaling through a Runx2 functional domain during osteoblast differentiation [J].

Bae, Jong-Sup ;

Gutierrez, Soraya ;

Narla, Radhika ;

Pratap, Jitesh ;

Devados, Rajitha ;

van Wijnen, Andre J. ;

Stein, Janet L. ;

Stein, Gary S. ;

Lian, Jane B. ;

Javed, Amjad .

JOURNAL OF CELLULAR BIOCHEMISTRY, 2007, 100 (02) :434-449

[2]

Calkhoven CF, 2000, GENE DEV, V14, P1920

[3]

C/EBPβ and its binding element are required for NFκB-induced COX2 expression following hypertonic stress [J].

Chen, J ;

Zhao, M ;

Rao, R ;

Inoue, H ;

Hao, CM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (16) :16354-16359

[4]

SWI/SNF-Independent Nuclease Hypersensitivity and an Increased Level of Histone Acetylation at the P1 Promoter Accompany Active Transcription of the Bone Master Gene Runx2 [J].

Cruzat, Fernando ;

Henriquez, Berta ;

Villagra, Alejandro ;

Hepp, Matias ;

Lian, Jane B. ;

van Wijnen, Andre J. ;

Stein, Janet L. ;

Imbalzano, Anthony N. ;

Stein, Gary S. ;

Montecino, Martin .

BIOCHEMISTRY, 2009, 48 (30) :7287-7295

[5]

Functional cooperation between CCAAT/enhancer-binding proteins and the vitamin D receptor in regulation of 25-hydroxyvitamin D324-hydroxylase [J].

Dhawan, P ;

Peng, XR ;

Sutton, ALM ;

MacDonald, PN ;

Croniger, CM ;

Trautwein, C ;

Centrella, M ;

McCarthy, TL ;

Christakos, S .

MOLECULAR AND CELLULAR BIOLOGY, 2005, 25 (01) :472-487

[6]

Drissi H, 2000, J CELL PHYSIOL, V184, P341, DOI 10.1002/1097-4652(200009)184:3<341::AID-JCP8>3.0.CO

[7]

2-Z

[8]

Canonical WNT signaling promotes osteogenesis by directly stimulating Runx2 gene expression [J].

Gaur, T ;

Lengner, CJ ;

Hovhannisyan, H ;

Bhat, RA ;

Bodine, PVN ;

Komm, BS ;

Javed, A ;

van Wijnen, AJ ;

Stein, JL ;

Stein, GS ;

Lian, JB .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (39) :33132-33140

[9]

CCAAT/enhancer-binding proteins (C/EBP) β and δ activate osteocalcin gene transcription and synergize with Runx2 at the C/EBP element to regulate bone-specific expression [J].

Gutierrez, S ;

Javed, A ;

Tennant, DK ;

van Rees, M ;

Montecino, M ;

Stein, GS ;

Stein, JL ;

Lian, JB .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (02) :1316-1323

[10]

Col1a1 promoter-targeted expression of p20 CCAAT enhancer-binding protein β (C/EBPβ), a truncated c/EBPβ isoform, causes osteopenia in transgenic mice [J].

Harrison, JR ;

Huang, YF ;

Wilson, KA ;

Kelly, PL ;

Adams, DJ ;

Gronowicz, GA ;

Clark, SH .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (09) :8117-8124

← 1 2 3 4 5 →