Ralstonia metallidurans, a bacterium specifically adapted to toxic metals:: towards a catalogue of metal-responsive genes

被引:312
作者
Mergeay, M [1 ]
Monchy, S
Vallaeys, T
Auquier, V
Benotmane, A
Bertin, P
Taghavi, S
Dunn, J
van der Lelie, D
Wattiez, R
机构
[1] CEN SCK, Belgium Nucl Res Ctr, Labs Microbiol & Radiobiol, B-2400 Mol, Belgium
[2] Flemish Inst Technol Res, Environm Technol Expertise Ctr, B-2400 Mol, Belgium
[3] Free Univ Brussels, Unite Etud Conformat Macromol Biol, B-1050 Brussels, Belgium
[4] INRA, Dept Microbiol, Jouy En Josas, France
[5] Free Univ Brussels, Ctr Biol Struct & Bioinformat Struct & Fonct Memb, B-1050 Brussels, Belgium
[6] Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA
[7] Univ Mons, Serv Chim Biol, B-7000 Mons, Belgium
关键词
Ralstonia metallidurans; heavy metal; resistance gene; Pl-ATPase; genome; proteomics; HME-RND efflux system; plasmid; mobile genetic; element; regulation;
D O I
10.1016/S0168-6445(03)00045-7
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Ralstonia metallidurans, formerly known as Alcaligenes eutrophus and thereafter as Ralstonia eutropha, is a beta-Proteobacterium colonizing industrial sediments, soils or wastes with a high content of heavy metals. The type strain CH34 carries two large plasmids (pMOL28 and pMOL30) bearing a variety of genes for metal resistance. A chronological overview describes the progress made in the knowledge of the plasmid-borne metal resistance mechanisms, the genetics of R. metallidurans CH34 and its taxonomy, and the applications of this strain in the fields of environmental remediation and microbial ecology. Recently, the sequence draft of the genome of R. metallidurans has become available. This allowed a comparison of these preliminary data with the published genome data of the plant pathogen Ralstonia solanacearum, which harbors a megaplasmid (of 2.1 Mb) carrying some metal resistance genes that are similar to those found in R. metallidurans CH34. In addition, a first inventory of metal resistance genes and operons across these two organisms could be made. This inventory, which partly relied on the use of proteomic approaches, revealed the presence of numerous loci not only on the large plasmids pMOL28 and pMOL30 but also on the chromosome. It suggests that metal-resistant Ralstonia, through evolution, are particularly well adapted to the harsh environments typically created by extreme anthropogenic situations or biotopes. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
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页码:385 / 410
页数:26
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