Rapid purification of two thermophilic proteinases using dye-ligand chromatography

被引：5

作者：

Cowan, DA ^{[1
]}

Daniel, RM ^{[1
]}

机构：

[1] UNIV WAIKATO,THERMOPHILE RES UNIT,HAMILTON,NEW ZEALAND

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1996年 / 32卷 / 01期

关键词：

EXTRACELLULAR PROTEINASE; ESCHERICHIA-COLI; THERMUS-SP; SEPHAROSE; DEHYDROGENASE; KINASE; STRAIN;

D O I：

10.1016/0165-022X(95)00024-L

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Dye-ligand chromatography has been used successfully for the purification of extracellular thermostable proteinases from thermophilic Bacillus and Thermus cultures. Single-step purification factors of up to 115-fold (for Thermus protease) and 2195-fold (for Bacillus protease) were obtained, Elution studies suggested that the mode of binding involved the enzyme active sites. The method was readily scalable to 600 1 volume.

引用

页码：1 / 6

页数：6

共 20 条

[1] FURTHER OBSERVATIONS ON DYE LIGAND AFFINITY-CHROMATOGRAPHY OF SERUM-ALBUMIN [J].

BARTON, JS .

JOURNAL OF CHEMICAL EDUCATION, 1985, 62 (05) :441-441

[2] F-0 MEMBRANE DOMAIN OF ATP SYNTHASE FROM BOVINE HEART-MITOCHONDRIA - PURIFICATION, SUBUNIT COMPOSITION, AND RECONSTITUTION WITH F1-ATPASE [J].

COLLINSON, IR ;

RUNSWICK, MJ ;

BUCHANAN, SK ;

FEARNLEY, IM ;

SKEHEL, JM ;

VANRAAIJ, MJ ;

GRIFFITHS, DE ;

WALKER, JE .

BIOCHEMISTRY, 1994, 33 (25) :7971-7978

[3] SCREENING OF STRAINS IDENTIFIED AS EXTREMELY THERMOPHILIC BACILLI FOR EXTRACELLULAR PROTEOLYTIC ACTIVITY AND GENERAL-PROPERTIES OF THE PROTEINASES FROM 2 OF THE STRAINS [J].

COOLBEAR, T ;

EAMES, CW ;

CASEY, Y ;

DANIEL, RM ;

MORGAN, HW .

JOURNAL OF APPLIED BACTERIOLOGY, 1991, 71 (03) :252-264

[4] PURIFICATION AND SOME PROPERTIES OF AN EXTRACELLULAR PROTEASE (CALDOLYSIN) FROM AN EXTREME THERMOPHILE [J].

COWAN, DA ;

DANIEL, RM .

BIOCHIMICA ET BIOPHYSICA ACTA, 1982, 705 (03) :293-305

[5] AN EXTREMELY THERMOSTABLE EXTRACELLULAR PROTEINASE FROM A STRAIN OF THE ARCHAEBACTERIUM DESULFUROCOCCUS GROWING AT 88-DEGREES-C [J].

COWAN, DA ;

SMOLENSKI, KA ;

DANIEL, RM ;

MORGAN, HW .

BIOCHEMICAL JOURNAL, 1987, 247 (01) :121-133

[6]

JANSSEN PH, 1991, APPL MICROBIOL BIOT, V34, P789

[7] PURIFICATION OF ASPARTASE AND ASPARTOKINASE HOMOSERINE DEHYDROGENASE-I FROM ESCHERICHIA-COLI BY DYE LIGAND CHROMATOGRAPHY [J].

KARSTEN, WE ;

HUNSLEY, JR ;

VIOLA, RE .

ANALYTICAL BIOCHEMISTRY, 1985, 147 (02) :336-341

[8]

KUTTY AVM, 1987, BIOCHEM ARCH, V3, P231

[9]

LOPES HR, 1993, REV MICROBIOL, V24, P228

[10] SIMULTANEOUS SEPARATION AND PURIFICATION OF PYRUVATE-KINASE AND LACTATE-DEHYDROGENASE BY DYE-LIGAND CHROMATOGRAPHY [J].

MAKRIYANNIS, T ;

CLONIS, YD .

PROCESS BIOCHEMISTRY, 1993, 28 (03) :179-185

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