Recommendations for calculating growth parameters by optical density measurements

被引：133

作者：

Begot, C ^{[1
]}

Desnier, I ^{[1
]}

Daudin, JD ^{[1
]}

Labadie, JC ^{[1
]}

Lebert, A ^{[1
]}

机构：

[1] NATL INST AGRON RES,F-63122 ST GENES CHAMPANE,FRANCE

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1996年 / 25卷 / 03期

关键词：

automated turbidimetry; bacterial growth; Listeria monocytogenes; optical density;

D O I：

10.1016/0167-7012(95)00090-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An automated turbidimetric system, Bioscreen C, was used to monitor bacterial growth in microplates. The detection threshold and linear range of the instrument were determined. Bioscreen C was found to present certain problems in setting up temperature and O.D. stability. A systematic way to calculate growth parameters (maximal growth rate, lag time and logarithmic increase of population) was developed. The repeatability of growth was studied. The validity of the method was illustrated by studying the growth of 9 strains of Listeria monocytogenes under two conditions: 37 degrees C, Aw 0.96, pH 7.0 and 10 degrees C, Aw 1.00, pH 7.0. Growths in Bioscreen C were compared with growth obtained in a fermenter.

引用

页码：225 / 232

页数：8

共 12 条

[1] EFFECT OF TEMPERATURE HISTORY ON THE GROWTH OF LISTERIA-MONOCYTOGENES SCOTT-A AT REFRIGERATION TEMPERATURES [J].

BUCHANAN, RL ;

KLAWITTER, LA .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1991, 12 (2-3) :235-246

[2] UNSATURATED SOLUTIONS OF SODIUM-CHLORIDE AS REFERENCE SOURCES OF WATER ACTIVITY AT VARIOUS TEMPERATURES [J].

CHIRIFE, J ;

RESNIK, SL .

JOURNAL OF FOOD SCIENCE, 1984, 49 (06) :1486-1488

[3] ESTIMATION OF BACTERIAL-GROWTH RATES FROM TURBIDIMETRIC AND VIABLE COUNT DATA [J].

DALGAARD, P ;

ROSS, T ;

KAMPERMAN, L ;

NEUMEYER, K ;

MCMEEKIN, TA .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1994, 23 (3-4) :391-404

[4]

FOURNAUD J, 1973, 19 REUN EUR CHERCH V

[5] PSYCHROPHILIC AND PSYCHROTROPHIC MICROORGANISMS [J].

GOUNOT, AM .

EXPERIENTIA, 1986, 42 (11-12) :1192-1197

[6] EFFECT OF GROWTH TEMPERATURES ON THE PROTEIN-LEVELS IN A PSYCHROTROPHIC BACTERIUM, PSEUDOMONAS-FRAGI [J].

HEBRAUD, M ;

DUBOIS, E ;

POTIER, P ;

LABADIE, J .

JOURNAL OF BACTERIOLOGY, 1994, 176 (13) :4017-4024

[7] COMPARISON OF RESPONSE-SURFACE MODELS FOR LISTERIA-MONOCYTOGENES STRAINS UNDER AEROBIC CONDITIONS [J].

HUDSON, JA .

FOOD RESEARCH INTERNATIONAL, 1994, 27 (01) :53-59

[8] THE USE OF AUTOMATED TUBIDIMETRIC DATA FOR THE CONSTRUCTION OF KINETIC-MODELS [J].

MCCLURE, PJ ;

COLE, MB ;

DAVIES, KW ;

ANDERSON, WA .

JOURNAL OF INDUSTRIAL MICROBIOLOGY, 1993, 12 (3-5) :277-285

[9] A STUDY OF FACTORS AFFECTING GROWTH AND RECOVERY OF LISTERIA-MONOCYTOGENES SCOTT,A [J].

PETRAN, RL ;

ZOTTOLA, EA .

JOURNAL OF FOOD SCIENCE, 1989, 54 (02) :458-460

[10] GROWTH OF LISTERIA-MONOCYTOGENES AT REFRIGERATION TEMPERATURES [J].

WALKER, SJ ;

ARCHER, P ;

BANKS, JG .

JOURNAL OF APPLIED BACTERIOLOGY, 1990, 68 (02) :157-162

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