LPS-induced immune response in Drosophila

被引:14
作者
Imler, JL
Tauszig, S
Jouanguy, E
Forestier, C
Hoffmann, JA
机构
[1] CNRS, Inst Biol Mol & Cellulaire, UPR9022, F-67084 Strasbourg, France
[2] CIML, Marseille, France
来源
JOURNAL OF ENDOTOXIN RESEARCH | 2000年 / 6卷 / 06期
关键词
D O I
10.1179/096805100101532423
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The study of the regulation of the inducible synthesis of antimicrobial peptides in Drosophila melanogaster has established this insect as a powerful model in which to study innate immunity. In particular, the molecular characterization of the regulatory pathway controlling the antifungal peptide drosomycin has revealed the importance of Toll receptors in innate immunity. We report here that injection of LPS into flies induces an immune response, suggesting that LPS receptors are used in Drosophila to detect Gram-negative bacteria infection. We have identified in the recently sequenced genome of Drosophila eight genes coding for Toll-like;e receptors in addition to Toll, which may function as LPS receptors. However, overexpression of a selection of these genes in tissue-culture cells does not result in up-regulation of the antibacterial peptide genes. These results are discussed in light of the recent data from genetic screens aimed at identifying the genes controlling the antibacterial response in Drosophila.
引用
收藏
页码:459 / 462
页数:4
相关论文
共 17 条
[1]   Drosophila cecropin as an antifungal agent [J].
Ekengren, S ;
Hultmark, D .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1999, 29 (11) :965-972
[2]  
ELDON E, 1994, DEVELOPMENT, V120, P885
[3]  
GERTTULA S, 1988, GENETICS, V119, P123
[4]   Relish, a central factor in the control of humoral but not cellular immunity in Drosophila [J].
Hedengren, M ;
Åsling, B ;
Dushay, MS ;
Ando, I ;
Ekengren, S ;
Wihlborg, M ;
Hultmark, D .
MOLECULAR CELL, 1999, 4 (05) :827-837
[5]   Drosophila immunity [J].
Hoffmann, JA ;
Reichhart, JM .
TRENDS IN CELL BIOLOGY, 1997, 7 (08) :309-316
[6]   Signaling mechanisms in the antimicrobial host defense of Drosophila [J].
Imler, JL ;
Hoffmann, JA .
CURRENT OPINION IN MICROBIOLOGY, 2000, 3 (01) :16-22
[7]   The beginning of the end:: IκB kinase (IKK) and NF-κB activation [J].
Karin, M .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (39) :27339-27342
[8]   A RECESSIVE MUTATION, IMMUNE-DEFICIENCY (IMD), DEFINES 2 DISTINCT CONTROL PATHWAYS IN THE DROSOPHILA HOST-DEFENSE [J].
LEMAITRE, B ;
KROMERMETZGER, E ;
MICHAUT, L ;
NICOLAS, E ;
MEISTER, M ;
GEORGEL, P ;
REICHHART, JM ;
HOFFMANN, JA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (21) :9465-9469
[9]   The Drosophila caspase Dredd is required to resist Gram-negative bacterial infection [J].
Leulier, F ;
Rodriguez, A ;
Khush, RS ;
Abrams, JM ;
Lemaitre, B .
EMBO REPORTS, 2000, 1 (04) :353-358
[10]  
LU Y, 2000, GENE DEV, V15, P104