The identification and differential expression of calcium-binding proteins associated with ocular melanoma

被引:52
作者
Van Ginkel, PR
Gee, RL
Walker, TM
Hu, DN
Heizmann, CW
Polans, AS
机构
[1] Univ Wisconsin, Dept Ophthalmol & Visual Sci, Clin Sci Ctr K6-466, Madison, WI 53792 USA
[2] Univ Wisconsin, Dept Biomol Chem, Clin Sci Ctr K6-466, Madison, WI 53792 USA
[3] New York Eye & Ear Infirm, Tissue Culture Ctr, New York, NY 10003 USA
[4] New York Eye & Ear Infirm, Dept Pathol & Lab Med, New York, NY 10003 USA
[5] Univ Zurich, Dept Pediat, Div Clin Chem & Biochem, CH-8032 Zurich, Switzerland
来源
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1998年 / 1448卷 / 02期
关键词
calcium-binding proteins; S100; annexin; cap g; uveal melanoma;
D O I
10.1016/S0167-4889(98)00133-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Calcium-binding proteins may endow tumor cells with properties related to their malignancy and metastatic phenotype. Chromatographic procedures and amino acid sequence analysis were used in this study to identify seven calcium-binding proteins, annexiu. VI, cap g, annexin V, calmodulin, S100A11, S100B and S100A6, associated with uveal melanoma, the primary ocular tumor of adults. This series of calcium-binding proteins was identified in both primary tumors and cell lines of uveal melanoma. Several of the proteins were shown by immunochemical methods to be differentially expressed between normal uveal melanocytes and malignant melanomas of the uvea. In addition, the expression of S100A6 may correlate with the malignant properties of the tumor. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:290 / 297
页数:8
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